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A novel cell modification method used in biotransformation of glycerol to 3-HPA by Lactobacillus reuteri

  • Biotechnological products and process engineering
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Abstract

The aim of the present study was to develop a new cell modification method to facilitate the cell separation from broth. In order to reduce the transfer limitation of substrate and product caused by general immobilization methods in the following biotransformation of glycerol, the carboxyl-functioned superparamagnetic nanoparticle (MNP) was directly attached to the surface of Lactobacillus reuteri for 3-hydroxypropionealdehyde producing. The modification process could be finished in several minutes by just adding MNP fluid into the bulk fermentation broth. The modified cells could be rapidly separated from the solution with the aid of magnetic field. The interaction between cell and MNP was shown by electron microscopy. The efficiency of the cells attached by MNPs for transformation of various concentrations of glycerol (100–400 mM) was studied at various temperatures (25–40 °C) and pH levels (5.8–7.5) with different cell concentrations (7.5–30 g/L). The 3- hydroxypropionealdehyde (HPA)/glycerol molar conversion under optimal condition (30 °C and pH 7) reached 70 %. The inactive modified cell could be reactivated easily by fresh medium and recovered the ability of glycerol conversion. MNPS distributing on cell surface had little adverse effect on cell activity. The modification method simplified the two-step production of 3-HPA by resting L. reuteri. The method of MNPs attached to cell surface is totally different from the traditional immobilization method in which the cell is attached to or entrapped in big carrier. The results obtained in this study showed that carboxyl-functioned MNP could be directly used as cell modification particle and realized cell recycle with the aid of magnetic field in bioprocess.

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References

  • Chatzifragkou A, Papanikolaou S, Dietz D, Doulgeraki AI, Nychas GJE, Zeng AP (2011) Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process. Appl Microbiol Biot 91(1):101–112

    Article  CAS  Google Scholar 

  • Chen G, Fang BS (2011) Preparation of solid acid catalyst from glucose-starch mixture for biodiesel production. Bioresource Technol 102(3):2635–2640

    Article  CAS  Google Scholar 

  • Chen G, Ma YH, Su PF, Fang BS (2012) Direct binding glucoamylase onto carboxyl-functioned magnetic nanoparticles. Biochem Eng J 67:120–125

    Article  CAS  Google Scholar 

  • Chen G, Yang DM, Xiao YQ, Chen HW (2011a) Influence of conditions on reuterin accumulation by the resting cell biotransformation process. Chinese J Chem Eng 19(6):1023–1027

    Article  CAS  Google Scholar 

  • Chen G, Zhao J, Xiao YQ, Chen HW, Fang BS (2011b) Optimization of conditions for high production of 3-HPA through mathematical modelling of series reactions by resting Lactobacillus reuteri. Chem Biochem Eng Q 25(3):367–375

    Google Scholar 

  • Cho HY, Yousef AE, Yang ST (1996) Continuous production of pediocin by immobilized Pediococcus acidilactici P02 in a packed-bed bioreactor. Appl Microbiol Biot 45(5):589–594

    Article  CAS  Google Scholar 

  • Circle SJ, Stone L, Boruff CS (1945) Acrolein determination by means of tryptophane—a colorimetric micromethod. Ind Eng Chem Anal Ed 17(4):259–262

    Article  CAS  Google Scholar 

  • da Silva GP, Mack M, Contiero J (2009) Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv 27(1):30–39

    Article  Google Scholar 

  • Doleyres Y, Beck P, Vollenweider S, Lacroix C (2005) Production of 3-hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri. Appl Microbiol Biot 68(4):467–474

    Article  CAS  Google Scholar 

  • Hu ZC, Zheng YG, Shen YC (2011) Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor. Bioresource Technol 102(14):7177–7182

    Article  CAS  Google Scholar 

  • Kolesarova N, Hutnan M, Spalkova V, Kuffa R, Bodik I (2011) Anaerobic treatment of biodiesel by-products in a pilot scale reactor. Chem Pap 65(4):447–453

    Article  CAS  Google Scholar 

  • Krauter H, Willke T, Vorlop KD (2012) Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity. New Biotechnology 29(2):211–217

    Article  CAS  Google Scholar 

  • Lamers P, Hamelinck C, Junginger M, Faaij A (2011) International bioenergy trade-a review of past developments in the liquid biofuel market. Renew Sust Energ Rev 15(6):2655–2676

    Article  Google Scholar 

  • Luthi-Peng Q, Dileme FB, Puhan Z (2002a) Effect of glucose on glycerol bioconversion by Lactobacillus reuteri. Appl Microbiol Biot 59(2–3):289–296

    CAS  Google Scholar 

  • Luthi-Peng Q, Scharer S, Puhan Z (2002b) Production and stability of 3-hydroxypropionaldehyde in Lactobacillus reuteri. Appl Microbiol Biot 60(1–2):73–80

    CAS  Google Scholar 

  • Ngo TA, Kim MS, Sim SJ (2011) High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana. Int J Hydrogen Energ 36(10):5836–5842

    Article  CAS  Google Scholar 

  • Rutti DP, Lacroix C, Jeremic T, Mathis M, Die A, Vollenweider S (2011) Development of a reversible binding process for in situ removal of 3-hydroxypropionaldehyde during biotechnological conversion of glycerol. Biochem Eng J 55(3):176–184

    Article  Google Scholar 

  • Sindhu R, Ammu B, Binod P, Deepthi SK, Ramachandran KB, Soccol CR, Pandey A (2011) Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers. Braz Arch Biol Techn 54(4):783–794

    Article  CAS  Google Scholar 

  • Smiley KL, Sobolov M (1962) A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus. Arch Biochem Biophys 97(3):538

    Article  CAS  Google Scholar 

  • Stelmachowski M (2011) Utilization of glycerol, a by-product of the transesterification of vegetable oils: a review. Ecological Chemistry and Engineering S-Chemia I Inzynieria Ekologiczna S 18(1):9–30

    CAS  Google Scholar 

  • Su PF, Chen G, Zhao J (2011) Convenient preparation and characterization of surface carboxyl-functioned Fe3O4 magnetic nanoparticles. Chem J Chinese U 32(7):1472–1477

    CAS  Google Scholar 

  • Talarico TL, Dobrogosz WJ (1990) Purification and characterization of glycerol dehydratase from Lactobacillus reuteri. Appl Environ Microb 56(4):1195–1197

    CAS  Google Scholar 

  • Tobajas M, Mohedano AF, Casas JA, Rodriguez JJ (2007) A kinetic study of reuterin production by Lactobacillus reuteri PRO 137 in resting cells. Biochem Eng J 35(2):218–225

    Article  CAS  Google Scholar 

  • Tobimatsu T, Kajiura H, Yunoki M, Azuma M, Toraya T (1999) Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase. J Bacteriol 181(13):4110–4113

    CAS  Google Scholar 

  • Toraya T (2000) Radical catalysis of B-12 enzymes: structure, mechanism, inactivation, and reactivation of diol and glycerol dehydratases. Cell Mol Life Sci 57(1):106–127

    Article  CAS  Google Scholar 

  • Toraya T, Honda S, Mori K (2010) Coenzyme B-12-dependent diol dehydratase is a potassium ion-requiring calcium metalloenzyme: evidence that the substrate-coordinated metal ion is calcium. Biochemistry-us 49(33):7210–7217

    Article  CAS  Google Scholar 

  • Vollenweider S, Evers S, Zurbriggen K, Lacroix C (2010) Unraveling the hydroxypropionaldehyde (HPA) system: an active antimicrobial agent against human pathogens. J Agr Food Chem 58(19):10315–10322

    Article  CAS  Google Scholar 

  • Wang QZ, Ingram LO, Shanmugam KT (2011) Evolution of d-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for d-lactate production from lignocellulose. Proc Natl Acad Sci U S A 108(47):18920–18925

    Article  CAS  Google Scholar 

  • Zamudio-Jaramillo MA, Hernandez-Mendoza A, Robles VJ, Mendoza-Garcia PG, Espinosa-De-Los-Monteros JJ, Garcia HS (2009) Reuterin production by Lactobacillus reuteri NRRL B-14171 immobilized in alginate. J Chem Technol Biot 84(1):100–105

    Article  CAS  Google Scholar 

  • Zhao YN, Chen G, Yao SJ (2006) Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae. Biochem Eng J 32(2):93–99

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors express their thanks for the support from the Nature Science Foundation of China (20906035) and the Fundamental Research Funds for Huaqiao University (JB-SJ1008).

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Authors and Affiliations

  1. Department of Bioengineering and Biotechnolgy, Huaqiao University, Jimei Ave. 668, Xiamen, 361021, People’s Republic of China

    Guo Chen & Jialu Chen

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  1. Guo Chen
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  2. Jialu Chen
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Correspondence to Guo Chen.

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Chen, G., Chen, J. A novel cell modification method used in biotransformation of glycerol to 3-HPA by Lactobacillus reuteri . Appl Microbiol Biotechnol 97, 4325–4332 (2013). https://doi.org/10.1007/s00253-013-4723-2

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