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Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures

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Abstract

Long-term stable cell growth and production of vindoline, catharanthine, and ajmalicine of cambial meristematic cells (CMCs) from Catharanthus roseus were observed after 2 years of culture. C. roseus CMCs were treated with β-cyclodextrin (β-CD) and methyl jasmonate (MeJA) individually or in combination and were cultured both in conventional Erlenmeyer flasks (100, 250, and 500 mL) and in a 5-L stirred hybrid airlift bioreactor. CMCs of C. roseus cultured in the bioreactor showed higher yields of vindoline, catharanthine, and ajmalicine than those cultured in flasks. CMCs of C. roseus cultured in the bioreactor and treated with 10 mM β-CD and 150 μM MeJA gave the highest yields of vindoline (7.45 mg/L), catharanthine (1.76 mg/L), and ajmalicine (58.98 mg/L), concentrations that were 799, 654, and 426 % higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. Quantitative reverse transcription (RT)-PCR showed that β-CD and MeJA upregulated transcription levels of genes related to the biosynthesis of terpenoid indole alkaloids (TIAs). This is the first study to report that β-CD induced the generation of NO, which plays an important role in mediating the production of TIAs in C. roseus CMCs. These results suggest that β-CD and MeJA can enhance the production of TIAs in CMCs of C. roseus, and thus, CMCs of C. roseus have significant potential to be an industrial platform for production of bioactive alkaloids.

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Acknowledgments

This research work was financially supported by National Natural Sciences Foundation of China (No. 81073004, 81102771, and 81274045), Pearl River Scientific and Technological New Star Program of Guangzhou (No. 2014 J2200004), the Natural Science Foundation of Guangdong Province (No. 2014A030313385), and Science and Technology Specific Project of Guangzhou (No.201300000138).

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    Authors and Affiliations

    1. Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou, 510632, China

      Pengfei Zhou, Jiazeng Yang, Shuijie He, Wenjin Zhang, Rongmin Yu, Jiachen Zi & Liyan Song

    2. Department of Natural Products Chemistry, College of Pharmacy, Jinan University, Guangzhou, 510632, China

      Jianhua Zhu & Rongmin Yu

    3. Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou, 510632, China

      Xuesong Huang

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    Correspondence to Jianhua Zhu, Rongmin Yu or Xuesong Huang.

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    Pengfei Zhou and Jiazeng Yang contributed equally to this work.

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    Zhou, P., Yang, J., Zhu, J. et al. Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures. Appl Microbiol Biotechnol 99, 7035–7045 (2015). https://doi.org/10.1007/s00253-015-6651-9

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