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The effect of tryptophol on the bacteriophage infection in high-temperature environment

  • Applied microbial and cell physiology
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Abstract

Small metabolites can participate in the virus-host interactions in eukaryotes. However, little is known about roles of metabolites in the interactions between bacteria and bacteriophages. In this study, the metabolomic profilings of bacteriophage GVE2-infected and virus-free Geobacillus sp. E263, a thermophilic bacterium isolated from a deep-sea hydrothermal vent, were characterized. The results showed that metabolites tryptophol, adenine, and hydroxybenzylalcohol were significantly elevated in Geobacillus sp. E263 in response to the GVE2 infection. Furthermore, our data indicated that tryptophol was involved in the bacteriophage infection. Tryptophol could inhibit the infection/replication of GVE2 by interacting with the host’s Clp protease. Therefore, our study revealed novel aspects of metabolites during the bacteriophage infection in high-temperature environment.

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Acknowledgments

This work was financially supported by China Ocean Mineral Resources R & D Association (DY125-15-E-01), the National Natural Science Foundation of China (41276152), and Hi-Tech Research and Development Program of China (2012AA092103-5).

Conflict of interest

All the authors declared that they have no competing interest.

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Authors and Affiliations

  1. Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Min Jin, Chenxi Xu & Xiaobo Zhang

  2. State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen, 361005, China

    Min Jin

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  1. Min Jin
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  2. Chenxi Xu
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  3. Xiaobo Zhang
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Correspondence to Xiaobo Zhang.

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Jin, M., Xu, C. & Zhang, X. The effect of tryptophol on the bacteriophage infection in high-temperature environment. Appl Microbiol Biotechnol 99, 8101–8111 (2015). https://doi.org/10.1007/s00253-015-6674-2

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  • DOI: https://doi.org/10.1007/s00253-015-6674-2

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