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Enhanced reduction of Fe(III) oxides and methyl orange by Klebsiella oxytoca in presence of anthraquinone-2-disulfonate

  • Environmental biotechnology
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Abstract

Klebsiella oxytoca GS-4-08 is capable of azo dye reduction, but its quinone respiration and Fe(III) reduction abilities have not been reported so far. In this study, the abilities of this strain were reported in detail for the first time. As the biotic reduction of Fe(III) plays an important role in the biogeochemical cycles, two amorphous Fe(III) oxides were tested as the sole electron acceptor during the anaerobic respiration of strain GS-4-08. For the reduction of goethite and hematite, the biogenic Fe(II) concentrations reached 0.06 and 0.15 mM, respectively. Humic acid analog anthraquinone-2-disulfonate (AQS) was found to serve as an electron shuttle to increase the reduction of both methyl orange (MO) and amorphous Fe(III) oxides, and improve the dye tolerance of the strain. However, the formation of Fe(II) was not accelerated by biologically reduced AQS (B-AH2QS) because of the high bioavailability of soluble Fe(III). For the K. oxytoca strain, high soluble Fe(III) concentrations (above 1 mM) limit its growth and decolorization ability, while lower soluble Fe(III) concentrations produce an electron competition with MO initially, and then stimulate the decolorization after the electron couples of Fe(III)/Fe(II) are formed. With the ability to respire both soluble Fe(III) and insoluble Fe(III) oxides, this formerly known azo-reducer may be used as a promising model organism for the study of the interaction of these potentially competing processes in contaminated environments.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 51308300), Natural Science Foundation of Jiangsu Province (CN) (BK20151518), and partially funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Open Project of Jiangsu Key Laboratory of Biomass Energy and Materials (JSBEM201506).

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    Authors and Affiliations

    1. College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China

      Lei Yu, Shi Wang, Qing-wen Tang, Ming-yue Cao, Jia Li, Kun Yuan & Ping Wang

    2. Jiangsu Key Laboratory of Biomass Energy and Materials, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, 210042, China

      Lei Yu & Ming-yue Cao

    3. CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China

      Wen-wei Li

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    1. Lei Yu
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    Correspondence to Lei Yu.

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    Lei Yu and Shi Wang contributed equally to this work.

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    Yu, L., Wang, S., Tang, Qw. et al. Enhanced reduction of Fe(III) oxides and methyl orange by Klebsiella oxytoca in presence of anthraquinone-2-disulfonate. Appl Microbiol Biotechnol 100, 4617–4625 (2016). https://doi.org/10.1007/s00253-016-7281-6

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