Abstract
Persistent use of the diphenyl ether herbicides oxyfluorfen may seriously increase the health risks and ecological safety problems. A newly bacterium R-21 isolated from active soil was able to degrade and utilize oxyfluorfen as the sole carbon source. R-21 was identified as Chryseobacterium aquifrigidense by morphology, physiobiochemical characteristics, and genetic analysis. Under the optimum cultural conditions (pH 6.9, temperature 33.4 °C, and inoculum size 0.2 g L−1), R-21 could degrade 92.1 % of oxyfluorfen at 50 mg L−1 within 5 days. During oxyfluorfen degradation, six metabolites were detected and identified by atmospheric pressure gas chromatography coupled to quadrupole–time of flight mass spectrometry and ultra-performance liquid chromatography coupled to quadrupole–time of flight mass spectrometry, and a plausible degradation pathway was deduced. Strain R-21 is a promising potential in bioremediation of oxyfluorfen-contaminated environments.
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This study was funded by the National Natural Science Foundation of China (31171879, 31371968).
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Zhao, H., Xu, J., Dong, F. et al. Characterization of a novel oxyfluorfen-degrading bacterial strain Chryseobacterium aquifrigidense and its biochemical degradation pathway. Appl Microbiol Biotechnol 100, 6837–6845 (2016). https://doi.org/10.1007/s00253-016-7504-x
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DOI: https://doi.org/10.1007/s00253-016-7504-x