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Inhibition of Cyanide-Insensitive Respiration in Klebsiella oxytoca SYSU-011 by 8-Hydroxyquinolone

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Abstract

The inhibition of the cyanide (KCN)-insensitive respiration of Klebsiella oxytoca SYSU-011 by 8-hydroxyquinoline (8-HQ) was determined. Results showed that the profile of the rate of oxygen uptake of normal-grown and 8-HQ–grown K. oxytoca SYSU-011 was biphasic and similar, suggesting that 8-HQ did not inhibit the respiration of normal-grown K. oxytoca SYSU-011. A different biphasic KCN inhibition profile of respiration was observed for KCN-grown cells treated with and without 8-HQ. No decrease in respiration rate of KCN-grown cells and a 40% decrease in respiration rate of KCN-grown cells treated with 8-HQ were observed when KCN concentration was 10–1 mM. Comparing differences of the profiles of oxygen uptake in KCN-grown cells with and without 8-HQ addition indicated that 8-HQ inhibited expression of the KCN-insensitive pathway carried out by nonheme oxidase. Greater inhibition of NADH oxidase activity by 2-n-heptyl-4-hydroxyquinoline-N-oxide from the cell membrane of the KCN-grown cells treated with 8-HQ, and more H2O2 production from these cells with than without 8-HQ, suggest that the function of the cyanide-insensitive pathway can stabilize the respiration of the cyanide-grown cells to prevent the production of H2O2.

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Acknowledgments

This project was funded by Taiwan National Science Council (NSC 93-2314-B-017-001).

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Authors and Affiliations

  1. Institute of Environmental Engineering, National SunYat-Sen University, Kaohsiung, Taiwan, ROC

    Chih Ming Kao

  2. Department of Cosmeceutics, China Medical University, Taichung, Taiwan, ROC

    You Cheng Hseu

  3. Department of Biologic Science, National SunYat-Sen University, Kaohsiung, Taiwan, ROC

    Yen Ling Huang

  4. Bioinformatics Center, Chang Gung University, Taoyuan, Taiwan, ROC

    Peturs Tang

  5. Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan, ROC

    Ssu Ching Chen

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  1. Chih Ming Kao
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  2. You Cheng Hseu
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Correspondence to Ssu Ching Chen.

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Kao, C.M., Hseu, Y.C., Huang, Y.L. et al. Inhibition of Cyanide-Insensitive Respiration in Klebsiella oxytoca SYSU-011 by 8-Hydroxyquinolone. Curr Microbiol 54, 190–194 (2007). https://doi.org/10.1007/s00284-006-0231-2

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  • DOI: https://doi.org/10.1007/s00284-006-0231-2

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