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Pathway for Degradation of 2-Chloro-4-Nitrophenol in Arthrobacter sp. SJCon

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Abstract

Degradation of 2-Chloro-4-nitrophenol (2C4NP) was studied by Arthrobacter sp. SJCon, isolated from the soil of a pesticide contaminated site. This strain utilized 2C4NP as sole source of carbon and energy and degraded 2C4NP with stoichiometric release of nitrite and chloride ions. A metabolite was detected during the study of 2C4NP degradation and identified as chlorohydroquinone (CHQ) by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC–MS). Inhibition study using 2,2′-dipyridyl showed that CHQ is a terminal aromatic compound in degradation pathway of 2C4NP. CHQ dioxygenase activity was observed in the crude extract of 2C4NP induced cells of the strain SJCon that suggested the cleavage of the CHQ to maleylacetate (MA). Our study clearly showed that Arthrobacter sp. SJCon degraded 2C4NP via formation of CHQ that further cleaved to MA by CHQ dioxygenase. This mechanism of degradation of 2C4NP differs from previously reported degradation pathways of 2C4NP.

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Acknowledgments

PKA is thankful to the University Grants Commission for Dr. D. S. Kothari Postdoctoral Fellowship and the Council of Scientific and Industrial Research (CSIR), Govt. of India for Junior and Senior Research Fellowships.

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  1. Pankaj Kumar Arora

    Present address: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

Authors and Affiliations

  1. Environmental Biotechnology, Institute of Microbial Technology (CSIR), Sector-39A, Chandigarh, 160036, India

    Pankaj Kumar Arora & Rakesh Kumar Jain

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  1. Pankaj Kumar Arora
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  2. Rakesh Kumar Jain
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Correspondence to Pankaj Kumar Arora.

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Rakesh Kumar Jain—Deceased.

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Arora, P.K., Jain, R.K. Pathway for Degradation of 2-Chloro-4-Nitrophenol in Arthrobacter sp. SJCon. Curr Microbiol 63, 568 (2011). https://doi.org/10.1007/s00284-011-0022-2

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  • DOI: https://doi.org/10.1007/s00284-011-0022-2

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