Abstract
A fruity aroma-producing strain WG4 was isolated from a water sample collected from the Western Ghats, India. The 16S rRNA gene sequence analysis of strain WG4 indicated that Chryseobacterium indologenes, a member of the family ‘Flavobacteriaceae’ is the closest related species with a pair-wise sequence similarity of 98.6%. Strain WG4 produces a fruity aroma when grown on nutrient or trypticase soy agar plates. The fruity aroma is more when the strain WG4 is grown on agar plates compared to their growth in broth. The aromatic compounds produced by the strain WG4 were identified as ester compounds and were confirmed as ethyl-2-methylbutyrate and ethyl-3-methylbutyrate based on Gas Chromatography–Mass Spectrometry (GC–MS) analysis and using standard reference compounds. Even after repeated subcultures strain WG4 produced the same aroma in high intensity. Thus, strain WG4 could serve as a source for the production of these flavour compounds.
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Acknowledgments
We would like to thank Ram, M. S. from CCMB, Hyderabad, India for providing the sample. SS is thankful to CSIR Network Project on Exploitation of India’s rich microbial diversity (NWP0006) for funding. TNRS acknowledges the CSIR, Government of India for the award of Research Associateship.
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284_2011_9966_MOESM1_ESM.ppt
Supplementary Fig. 1 Phylogenetic tree based on 16S rRNA gene sequences showing the relationship of Chryseobacterium sp. strain WG4 with the closely related members of the genus Chryseobacterium. Phylogenetic tree was constructed using the Maximum likelihood method. Elizabethkingia miricola GTC862T was used as an out group. Numbers at nodes are bootstrap values. The bar represents 0.01 substitutions per alignment position. (PPT 115 kb)
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Kumar, P.A., Srinivas, T.N.R., Prasad, A.R. et al. Identification of Fruity Aroma-Producing Compounds from Chryseobacterium sp. Isolated from the Western Ghats, India. Curr Microbiol 63, 193–197 (2011). https://doi.org/10.1007/s00284-011-9966-5
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DOI: https://doi.org/10.1007/s00284-011-9966-5