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Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production

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Abstract

The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. oryzae BCC7051. The obtained reads were de novo assembled into 25 scaffolds spanning of 38,550,958 bps with predicted 11,456 protein-coding genes. By synteny mapping, a large rearrangement was found in two scaffolds of A. oryzae BCC7051 as compared to the reference RIB40 strain. The genetic relationship between BCC7051 and other strains of A. oryzae in terms of aflatoxin production was investigated, indicating that the A. oryzae BCC7051 was categorized into group 2 nonaflatoxin-producing strain. Moreover, a comparative analysis of the structural genes focusing on the involvement in lipid metabolism among oleaginous yeast and fungi revealed the presence of multiple isoforms of metabolic enzymes responsible for fatty acid synthesis in BCC7051. The alternative routes of acetyl-CoA generation as oleaginous features and malate/citrate/pyruvate shuttle were also identified in this A. oryzae strain. The genome sequence generated in this work is a dedicated resource for expanding genome-wide study of microbial lipids at systems level, and developing the fungal-based platform for production of diversified lipids with commercial relevance.

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Acknowledgements

We gratefully thank Uppsala Genome Center for Pacific BioScience platform sequencing service. We also thank Comparative Genomics Group, Division of BioScience, Oak Ridge National Laboratory for computational resources for gene prediction.

Funding

This work was financially supported by National Center for Genetic Engineering and Biotechnology (Project No. P-14-50613). The Swedish Research Council (VR-2013-4504) is acknowledged for financial support of Intawat Nookaew and sequencing.

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Authors and Affiliations

  1. Biochemical Engineering and Pilot Plant Research and Development Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand

    Chinae Thammarongtham & Tayvich Vorapreeda

  2. Department of Biomedical Informatics, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA

    Intawat Nookaew

  3. Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    Intawat Nookaew

  4. Comparative Genomics Group, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    Intawat Nookaew & Miriam L. Land

  5. Systems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand

    Tanawut Srisuk

  6. Bioprocess Technology Laboratory, Food Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, 12120, Thailand

    Sukanya Jeennor & Kobkul Laoteng

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  1. Chinae Thammarongtham
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Correspondence to Kobkul Laoteng.

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Thammarongtham, C., Nookaew, I., Vorapreeda, T. et al. Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production. Curr Microbiol 75, 57–70 (2018). https://doi.org/10.1007/s00284-017-1350-7

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