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The Plant Growth-Promoting Bacteria Strain Bacillus mojavensis I4 Enhanced Salt Stress Tolerance in Durum Wheat

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Abstract

Plant growth and production are adversely affected by soil salinity. A plant growth-promoting bacteria (PGPB) designated as the “I4 strain” of Bacillus mojavensis was isolated from Tunisian soil (Sfax, Tunisia) and showed the ability to be grown in the presence of NaCl concentrations ranging from 0 to 10% in Luria Bertani (LB) medium. The PGPB-mediated salt tolerance in durum wheat was evaluated. The physiological parameters such as growth, shoot and root length, dry and fresh weight were higher in I4-inoculated wheat plants in comparison with non-treated plants under salt stress. Results showed that this strain promoted wheat growth and preserved the membrane damage by notably lowering the electrolytes leakage and malondialdehyde content in contrast to non-inoculated plants. Moreover, leaf chlorophyll content, biochemical parameters and antioxidant enzyme activities measurement showed a better salt and heavy metal stress adaptation of the I4-inoculated plants. Due to these outcomes, it could be suggested that the inoculation of the PGPB I4 strain enhanced the wheat plant’s growth, especially under salt stress conditions. This study confirms the ameliorative role played by PGPB in tolerating salt stress in wheat and their potential use as biofertilizers to enhance its growth in saline soil and help in promoting this plant’s culture to provide food security under these perturbed global circumstances.

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Funding

This work was funded by the Ministry of Higher Education and Scientific Research-Tunisia.

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Authors and Affiliations

  1. Laboratory of Plant Improvement and Valorization of Agricultural Resources, National Engineering School of Sfax, Sfax, Tunisia

    Imen Ghazala, Nour Chiab & Radhia Gargouri-Bouzid

  2. Biotechnology and Plant Improvement Laboratory, Biotechnology Center of Sfax, Sfax, Tunisia

    Mohamed Najib Saidi

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  1. Imen Ghazala
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IG, NC and MNS conceived and designed the research. IG and NC conducted the experiments. IG, NC, MNS and RGB analyzed the data, contributed towards writing the manuscript, read and approved the manuscript.

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Correspondence to Imen Ghazala.

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Ghazala, I., Chiab, N., Saidi, M.N. et al. The Plant Growth-Promoting Bacteria Strain Bacillus mojavensis I4 Enhanced Salt Stress Tolerance in Durum Wheat. Curr Microbiol 80, 178 (2023). https://doi.org/10.1007/s00284-023-03288-y

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