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AoHapB, AoHapC and AoHapE, subunits of the Aspergillus oryzae CCAAT-binding complex, are functionally interchangeable with the corresponding subunits in Aspergillus nidulans

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Abstract.

Two genes, AohapB and AohapE, encoding subunits of the Aspergillus oryzae CCAAT-binding complex were cloned and sequenced. The polypeptides encoded by AohapB and AohapE were expressed in Escherichia coli and used to reconstitute a DNA-binding complex with recombinant AoHapC. The DNA-binding activity was observed only in the presence of all three subunits, indicating that AoHapB, AoHapE and AoHapC are essential for CCAAT- binding. Furthermore, introduction of the AohapB, AohapC and AohapE genes into the A. nidulans hapB Δ, hapC Δ and hapE Δ strains, respectively, revealed that the A. oryzae Hap subunits are functionally interchangeable with the corresponding subunits in A. nidulans.

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Authors and Affiliations

  1. Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan, , , , ,

    Akimitsu Tanaka, Ken-ichi Kamei, Shinjiro Tanoue, Masashi Kato, Tetsuo Kobayashi & Norihiro Tsukagoshi

  2. Department of Genetics, University of Melbourne, Parkville, Victoria 3052, Australia, , , , ,

    Peter Papagiannopoulos, Meryl A. Davis & Michael J. Hynes

  3. Institut für Mikrobiologie und Genetik, Technische Universität Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany, , , , ,

    Stefan Steidl & Axel A. Brakhage

Authors
  1. Akimitsu Tanaka
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  2. Ken-ichi Kamei
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  3. Shinjiro Tanoue
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  4. Peter Papagiannopoulos
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  5. Stefan Steidl
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  6. Axel A. Brakhage
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  7. Meryl A. Davis
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  8. Michael J. Hynes
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  9. Masashi Kato
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  10. Tetsuo Kobayashi
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  11. Norihiro Tsukagoshi
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Tanaka, A., Kamei, Ki., Tanoue, S. et al. AoHapB, AoHapC and AoHapE, subunits of the Aspergillus oryzae CCAAT-binding complex, are functionally interchangeable with the corresponding subunits in Aspergillus nidulans. Curr Genet 39, 175–182 (2001). https://doi.org/10.1007/s002940100190

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  • DOI: https://doi.org/10.1007/s002940100190

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