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The inhibitory effect of disease-modifying anti-rheumatic drugs and steroids on gliostatin/platelet-derived endothelial cell growth factor production in human fibroblast-like synoviocytes

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Abstract

Gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) is known to have both angiogenic and arthritogenic activities. The purpose of this study was to investigate whether disease-modifying anti-rheumatic drugs (DMARDs) and steroids are involved in the regulation of GLS expression. Fibroblast-like synoviocytes (FLSs) obtained from patients with rheumatoid arthritis (RA) were cultured and stimulated by interleukin (IL)-1β with or without DMARDs and steroids. The expression levels of GLS were determined using the reverse transcription-polymerase chain reaction and an ELISA. In cultured rheumatoid FLSs, the expression of GLS mRNA was significantly increased by stimulation with IL-1β. By contrast, GLS mRNA levels in IL-1β-stimulated FLSs were reduced by treatment with aurothioglucose (AuTG) and dexamethasone (DEX). These findings indicate that AuTG and DEX have anti-rheumatic activity, which is mediated via the suppression of GLS production. Neither methotrexate (MTX) nor sulfasalazine (SSZ) had a significant influence on GLS levels in our study.

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Acknowledgements

This research was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science, and a Grant-in-Aid for Research from Nagoya City University, Japan.

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Authors and Affiliations

  1. Department of Musculoskeletal Medicine, Nagoya City University Graduate School of Medical Sciences, Mizuho-Ku, Nagoya , 467-8601, Japan

    Takuma Kusabe, Yuko Waguri-Nagaya, Tomohiro Tanikawa, Muneyoshi Fukuoka, Masaaki Kobayashi & Takanobu Otsuka

  2. Department of Molecular Neurobiology, Nagoya City University Graduate School of Medical Sciences, Mizuho-Ku, Nagoya , 467-8601, Japan

    Takuma Kusabe, Mineyoshi Aoyama & Kiyofumi Asai

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  1. Takuma Kusabe
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  2. Yuko Waguri-Nagaya
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  3. Tomohiro Tanikawa
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  8. Kiyofumi Asai
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Correspondence to Yuko Waguri-Nagaya.

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Kusabe, T., Waguri-Nagaya, Y., Tanikawa, T. et al. The inhibitory effect of disease-modifying anti-rheumatic drugs and steroids on gliostatin/platelet-derived endothelial cell growth factor production in human fibroblast-like synoviocytes. Rheumatol Int 25, 625–630 (2005). https://doi.org/10.1007/s00296-005-0624-8

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  • DOI: https://doi.org/10.1007/s00296-005-0624-8

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