Abstract
An in vitro propagation system for Artemisia judaica L., a traditional Egyptian medicinal plant, has been developed. De novo shoot organogenesis was induced by culturing etiolated hypocotyls and intact seedlings on medium supplemented with thidiazuron [N-phenyl-N′-(1,2,3-thidiazol-yl) urea] via callusing at the cotyledonary notch region. Up to 16 shoots formed per seedling cultured on a medium containing 1 µmol l−1 thidiazuron for an optimal duration of exposure of 20 days. Regenerated shoots formed roots when subcultured onto a medium containing 1 µmol l−1 indole-3-butyric acid. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. judaica.
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Abbreviations
- BAP :
-
Benzyladenine
- TDZ :
-
Thidiazuron
- IBA :
-
Indole-3-butyric acid
- NAA :
-
α-Naphthaleneacetic acid
- PGRs :
-
Plant growth regulators
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Acknowledgements
The authors are grateful for the collaboration and support of staff of the St. Katherine Protectorate, Sinai, Egypt. The financial support of the Natural Sciences and Engineering Research Council of Canada is gratefully acknowledged.
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Communicated by L.C. Fowke
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Liu, C.Z., Murch, S.J., EL-Demerdash, M. et al. Regeneration of the Egyptian medicinal plant Artemisia judaica L.. Plant Cell Rep 21, 525–530 (2003). https://doi.org/10.1007/s00299-002-0561-x
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DOI: https://doi.org/10.1007/s00299-002-0561-x