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Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures

  • Physiology and Biochemistry
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Abstract

Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100 μM) on day 6 and fed loganin (0.30 mM), tryptamine (0.15 mM), loganin plus tryptamine, or geraniol (0.1–1.0 mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition.

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Acknowledgements

This work was supported in part by the National Science Foundation (NSF-CAREER, Grant No. BES-0134511). A. Royce was supported in part by Giner, Inc. We would like to thank Dr. Robert Verpoorte (University of Leiden, The Netherlands) for the C. roseus cultures.

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  1. Chemical Engineering Department, 342 Snell Engineering Center, Northeastern University, 360 Huntington Avenue, Boston, MA, 02115-5000, USA

    Carolyn W. T. Lee-Parsons & Amber J. Royce

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  1. Carolyn W. T. Lee-Parsons
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  2. Amber J. Royce
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Correspondence to Carolyn W. T. Lee-Parsons.

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Communicated by K. K. Kamo

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Lee-Parsons, C.W.T., Royce, A.J. Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures. Plant Cell Rep 25, 607–612 (2006). https://doi.org/10.1007/s00299-005-0109-y

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  • DOI: https://doi.org/10.1007/s00299-005-0109-y

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