Abstract
Mitochondria in plant cells undergo fusion and fission frequently. Although the mechanisms and proteins of mitochondrial fusion are well known in yeast and mammalian cells, they remain poorly understood in plant cells. To clarify the physiological requirements for plant mitochondrial fusion, we investigated the fusion frequency of mitochondria in tobacco cultured cells using the photoconvertible fluorescent protein Kaede and some physiological inhibitors. The latter included two uncouplers, 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), an inhibitor of mitochondrial ATP synthase, oligomycin, and an actin polymerization inhibitor, latrunculin B (Lat B). The frequency of mitochondrial fusion was clearly reduced by DNP, CCCP and oligomycin, but not by Lat B, although Lat B severely inhibited mitochondrial movement. Moreover, DNP, CCCP and oligomycin evidently lowered the cellular ATP levels. These results indicate that plant mitochondrial fusion depends on the cellular ATP level, but not on actin polymerization.
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Abbreviations
- DNP:
-
2,4-Dinitrophenol
- CCCP:
-
Carbonyl cyanide m-chlorophenylhydrazone
- ΔΨm :
-
Mitochondrial inner membrane potential
- Lat B:
-
Latrunculin B
- GFP:
-
Green fluorescent protein
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Acknowledgments
We thank Dr M. Karimi (Ghent University, Belgium) for kindly donating Gateway destination vectors and Dr W. Sakamoto (Okayama University, Japan) for donating the Mt-GFP plasmid. This research was supported by Grants from the Nara Institute of Science and Technology (to KW) and the Ministry of Education, Culture, Sports, Science, and Technology of JAPAN (Grants 18075005 and 18208002 to NT, and Grant 17780002 to SA).
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Communicated by F. Brandizzi.
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Wakamatsu, K., Fujimoto, M., Nakazono, M. et al. Fusion of mitochondria in tobacco suspension cultured cells is dependent on the cellular ATP level but not on actin polymerization. Plant Cell Rep 29, 1139–1145 (2010). https://doi.org/10.1007/s00299-010-0897-6
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DOI: https://doi.org/10.1007/s00299-010-0897-6