Abstract
Aseptically propagated regenerates were cultivated in a hydroponic apparatus, a phytotron or in the field, and their growth and littorine contents were investigated. No littorine was detected in aseptic regenerates cultured on solidified Murashige and Skoog medium, nor was it found in leaves under the three conditions tested. In roots, it was common features to all three conditions tested that littorine increased dramatically after transplantation from culture tubes and was a major alkaloid up to week 4; subsequently the littorine contents varied depending on the cultivation conditions. Roots cultivated in the field showed a marked thickening and rapid disappearance of littorine; those cultivated in the hydroponic apparatus were thin and maintained a high level of littorine for a long time. In a plant cultivated for 16 weeks in a pot, littorine content in the roots decreased with increasing root diameter.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 1 October 1999 / Revision received: 16 March 2000 / Accepted: 24 March 2000
Rights and permissions
About this article
Cite this article
Nakanishi, F., Sasaki, K. & Shimomura, K. Kinetics of littorine content in various developing stages of regenerates of Atropa belladonna. Plant Cell Reports 19, 1021–1026 (2000). https://doi.org/10.1007/s002990000231
Issue Date:
DOI: https://doi.org/10.1007/s002990000231