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Abundance of transcripts of functional gene reflects the inverse relationship between CH4 and N2O emissions during mid-season drainage in acidic paddy soil

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Abstract

Agricultural management significantly affects methane (CH4) and nitrous oxide (N2O) emissions from paddy fields. However, little is known about the underlying microbiological mechanism. Field experiment was conducted to investigate the effect of the water regime and straw incorporation on CH4 and N2O emissions and soil properties. Quantitative PCR was applied to measure the abundance of soil methanogens, methane-oxidising bacteria, nitrifiers, and denitrifiers according to DNA and mRNA expression levels of microbial genes, including mcrA, pmoA, amoA, and nirK/nirS/nosZ. Field trials showed that the CH4 and N2O flux rates were negatively correlated with each other, and N2O emissions were far lower than CH4 emissions. Drainage and straw incorporation affected functional gene abundance through altered soil environment. The present (DNA-level) gene abundances of amoA, nosZ, and mcrA were higher with straw incorporation than those without straw incorporation, and they were positively correlated with high concentrations of soil exchangeable NH4+ and dissolved organic carbon. The active (mRNA-level) gene abundance of mcrA was lower in the drainage treatment than in continuous flooding, which was negatively correlated with soil redox potential (Eh). The CH4 flux rate was significantly and positively correlated with active mcrA abundance but negatively correlated with Eh. The N2O flux rate was significantly and positively correlated with present and active nirS abundance and positively correlated with soil Eh. Thus, we demonstrated that active gene abundance, such as of mcrA for CH4 and nirS for N2O, reflects the contradictory relationship between CH4 and N2O emissions regulated by soil Eh in acidic paddy soils.

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Acknowledgements

We would like to thank Editage [www.editage.cn] for English language editing.

Funding

This research was financially supported by the National Key Research and Development Program of China (2016YFD0200307), the National Natural Science Foundation of China (41771335, 41271280), and the Hunan Provincial Natural Science Foundation of China (2016JJ3133).

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Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Agro-Ecosystem Research Station, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China

    Hongling Qin, Jianlin Shen, Chunlan Chen, Jie Yang, Yi Liu, Xiangbi Chen, Yong Li & Haijun Hou

  2. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Life Science and Technology, Hubei Engineering University, Xiaogan, China

    Yafang Tang

  3. Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China

    Jianlin Shen, Xiangbi Chen & Yong Li

  4. Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou, 510650, China

    Cong Wang

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  1. Hongling Qin
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  2. Yafang Tang
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Yafang Tang contributed equally to this research.

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Correspondence to Haijun Hou.

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Qin, H., Tang, Y., Shen, J. et al. Abundance of transcripts of functional gene reflects the inverse relationship between CH4 and N2O emissions during mid-season drainage in acidic paddy soil. Biol Fertil Soils 54, 885–895 (2018). https://doi.org/10.1007/s00374-018-1312-7

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