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Physico-chemical stability and structural characterization of thickened multilamellar beta-carotene-loaded liposome dispersions produced using a proliposome method

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Abstract

The objective of this study was to investigate in more detail the structure of multilamellar beta-carotene-loaded liposome dispersions produced by proliposome hydration. Such dispersions were stabilized using xanthan gum as a thickener in different concentrations, and their stabilities were monitored for 90 days. The vesicles exhibited an average diameter in the range of 700 to 3000 nm, and the liposomes were capable of protecting β-carotene from degradation for a period of 90 days. The dispersions were also characterized by transmission electron microscopy, differential scanning calorimetry, rheology, and small-angle X-ray scattering (SAXS). The thermal analyses showed that neither the β-carotene nor the xanthan gum affected the liposome bilayer structure. The presence of the xanthan gum, which affects the scattering intensity, was not an obstacle to obtain the structural parameters by SAXS modeling, as a modified modeling strategy (Gaussian deconvolution) was applied. This modeling resulted in 40 symmetric layers, and the results obtained with the variation of temperature were in agreement with the gel-liquid crystalline transition temperature obtained by calorimetric measurements. Additionally, the rheological data showed that xanthan gum was not as effective as a mixture of xanthan gum and guar gum at stabilizing the liposomes, most likely due to the higher stiffness of the gum alone compared with that of its combination with guar gum.

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References

  1. Alves GP, Santana MHA (2004) Phospholipid dry powder produced by spray drying processing: structural, thermodynamic and physical properties. Powder Technol 145:139–148

    Article  CAS  Google Scholar 

  2. Cavalcanti LP, Torriani IL, Plivelic TS, Oliveira CLP, Kellermann G, Neuenschwander R (2004) Two new sealed sample cells for small angle x-ray scattering from macromolecules in solution and complex fluids using synchrotron radiation. Rev Sci Instrum 75:4541–4546

    Article  CAS  Google Scholar 

  3. De Paz E, Martín A, Cocero MJ (2012) Formulation of β-carotene with soybean lecithin by PGSS (particles from gas saturated solutions)-drying. J Supercrit Fluids 72:125–133

    Article  Google Scholar 

  4. Dickinson E (2009) Hydrocolloids as emulsifiers and emulsion stabilizers. Food Hydrocoll 23:1473–1482

    Article  CAS  Google Scholar 

  5. Ding B, Xia S, Hayat K, Zhang X (2009) Preparation and stability of ferrous glycinate liposomes. J Agric Food Chem 57:2938–2944

    Article  CAS  Google Scholar 

  6. Ding B, Zhang X, Hayat K, Xia S, Jia C, Xie M, Liu C (2011) Preparation, characterization and the stability of ferrous glycinate liposomes. J Food Eng 102:202–208

    Article  CAS  Google Scholar 

  7. Doublier JL, Cuvelier G (2006) Gums and hydrocolloids: functional aspects. In: Eliasson AC (ed) Carbohydrates in foods, 2nd edn. Taylor and Francis, Boca Raton, pp 233–271

    Google Scholar 

  8. Fraser PD, Bramley PM (2004) The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 43:228–265

    Article  CAS  Google Scholar 

  9. Garcia-Ochoa F, Santos VE, Casas JA, Gómez E (2000) Xanthan gum: production, recovery and properties. Biotechnol Adv 18:549–579

    Article  CAS  Google Scholar 

  10. Gibis M, Zeeb B, Weiss J (2014) Formation, characterization, and stability of encapsulated hibiscus extract in multilayered liposomes. Food Hydrocoll 38:28–39

    Article  CAS  Google Scholar 

  11. Gortzi O, Lalas S, Chinou I, Tsaknis J (2007) Evaluation of the antimicrobial and antioxidant activities of Origanum dictamnus extracts before and after encapsulation in liposomes. Molecules 12:932–945

    Article  CAS  Google Scholar 

  12. Gortzi O, Lalas S, Chinou I, Tsaknis J (2008) Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. Eur Food Res Technol 226:583–590

    Article  CAS  Google Scholar 

  13. Hammersley AP, Svensson SO, Hanfland M, Fitch AN, Hausermann D (1996) Two-dimensional detector software: from real detector to idealised image or two-theta scan. High Pressure Res 14:235–248

    Article  Google Scholar 

  14. Hasan M, Belhaj N, Benachour H, Barberi-heyob M, Kahn CJF, Jabbari E, Linder M, Arab-Tehrany E (2014) Liposome encapsulation of curcumin: physico-chemical characterizations and effects on MCF7 cancer cell proliferation. Int J Pharm 461:519–528

    Article  CAS  Google Scholar 

  15. Kikuchi H, Yamauchi H, Hirota S (1991) A spray drying method for the mass production of liposomes. Chem Pharm Bull 39:1522–1527

    Article  CAS  Google Scholar 

  16. Kucerka N, Nieh MP, Katsaras J (2010) Small-angle scattering from homogeneous and heterogeneous lipid bilayers. In: Iglic A, Tien HT (eds) Advances in planar lipid bilayers and liposomes, vol 12. Academic, Burlington, pp 201–236

    Chapter  Google Scholar 

  17. Laouini A, Jaafar-Maalej C, Limayen-Blouza I, Sfar S, Charcosset C, Fessi H (2012) Preparation, characterization and applications of liposomes: the state of art. J Colloid Sci Biotechnol 1:147–168

    Article  CAS  Google Scholar 

  18. Lu Q, Li D-C, Jiang J-G (2011) Preparation of a tea polyphenol nanoliposome system and its physicochemical properties. J Agric Food Chem 59:13004–13011

    Article  CAS  Google Scholar 

  19. Maherani B, Aab-Tehrany E, Kheirolomoom A, Cleymand F, Linder M (2012) Influence of lipid composition on physicochemical properties of nanoliposomes encapsulating natural dipeptide antioxidant l-carnosine. Food Chem 134:632–640

    Article  CAS  Google Scholar 

  20. Maherani B, Arab-Tehrany E, Mozafari MR, Gaiani C, Linder M (2011) Liposomes: a review of manufacturing techniques and targeting strategies. Curr Nanosci 7:436–452

    Article  CAS  Google Scholar 

  21. Marsanasco M, Márquez AL, Wagner JR, Alonso SV, Chiaramoni NS (2011) Liposomes as vehicles for vitamins E and C: an alternative to fortify orange juice and offer vitamin C protection against heat treatment. Food Res Int 44:3039–3046

    Article  CAS  Google Scholar 

  22. McClements DJ (2005) Food emulsions: principles, practice and techniques, 2nd edn. CRC, Washington

    Google Scholar 

  23. Milas M, Rinaudo M (1979) Conformational investigation on the bacterial polysaccharide xanthan. Carbohydr Res 76:189–196

    Article  CAS  Google Scholar 

  24. Moraes M, Carvalho JMP, Silva CR, Cho S, Sola MR, Pinho SC (2013) Liposomes encapsulating β-carotene produced by proliposomes method: characterization and shelf life of powders and phospholipid vesicles. Intl J Food Sci Technol 48:274–282

    Article  CAS  Google Scholar 

  25. Mozafari MR, Johnson C, Hatziantoniou S, Demetzos C (2008) Nanoliposomes and their applications in food nanotechnology. J Liposome Res 18:309–327

    Article  Google Scholar 

  26. Oliveira CLP, Gerbelli BB, Silva ERT, Nallet F, Navailles L, Oliveira EA, Pedersen JS (2012) Gaussian deconvolution: a useful method for a form-free modeling of scattering data from mono- and multilayered planar systems. J Appl Crystallogr 45:1278–1286

    Article  CAS  Google Scholar 

  27. Parada J, Aguilera JM (2007) Food microstructure affects the bioavailability of several nutrients. J Food Sci 72:21–32

    Article  Google Scholar 

  28. Patil YP, Jadhav S (2014) Novel methods for liposome preparation. Chem Phys Lipids 177:8–18

    Article  CAS  Google Scholar 

  29. Pintea A, Diehl HA, Momeu C, Aberle L, Socaciu C (2005) Incorporation of carotenoid esters into liposomes. Biophys Chem 118:7–14

    Article  CAS  Google Scholar 

  30. Rashidinejad A, Birch EJ, Sun-Waterhouse D, Everett DW (2014) Delivery of green tea catechin and epigallocatechin gallate in liposomes incorporated into low-fat hard cheese. Food Chem 156:176–183

    Article  CAS  Google Scholar 

  31. Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MNVR (2006) Role of antioxidants in prophylaxis and therapy: a pharmaceutical perspective. J Control Release 11:189–207

    Article  Google Scholar 

  32. Rocha FM, Pinho SC, Santana MHA, Zollner RL (2001) Preparation and characterization of affinity magnetoliposomes useful for detection of antiphospholipid antibodies. J Magn Magn Mater 225:101–108

    Article  CAS  Google Scholar 

  33. Socaciu C, Bojarski P, Aberle L, Diehl HA (2002) Different ways to insert carotenoids into liposomes affect structure and dynamics of the bilayer differently. Biophys Chem 99:1–15

    Article  CAS  Google Scholar 

  34. Socaciu C, Jessel R, Diehl HA (2000) Competitive carotenoid and cholesterol incorporation into liposomes: effects of membrane phase transition, fluidity, polarity and anisotropy. Chem Phys Lipids 106:79–88

    Article  CAS  Google Scholar 

  35. Tan C, Xia S, Xue J, Xie J, Feng B, Zhang X (2013) Liposomes as vehicles for lutein: preparation, stability, liposomal membrane dynamics, and structure. J Agric Food Chem 61:8175–8184

    Article  CAS  Google Scholar 

  36. Tan C, Xue J, Lou X, Abbas S, Guan Y, Feng B, Zhanga X, Xia S (2014) Liposomes as delivery systems for carotenoids: comparative studies of loading ability, storage stability and in vitro release. Food Funct 5:1232–1240

    Article  CAS  Google Scholar 

  37. Taylor TM, Weiss J, Davidson PM, Bruce BD (2005) Liposomal nanocapsules in food science and agriculture. Food Sci Nutr 45:587–605

    CAS  Google Scholar 

  38. Toniazzo T, Berbel IF, Cho S, Fávaro-Trindade CS, Moraes ICF, Pinho SC (2014) β-carotene-loaded liposome dispersions stabilized with xanthan and guar gums: physicochemical stability and feasibility of application in yogurt. LWT Food Sci Technol 59:1265–1273

    Article  CAS  Google Scholar 

  39. Van Vuuren SF, du Toit LC, Parry A, Pillay V, Choonara YE (2010) Encapsulation of essential oils within a polymeric liposomal formulation for enhancement of antimicrobial efficacy. Nat Prod Commun 5:1401–1409

    Google Scholar 

  40. Wagner A, Vorauer-Uhl K (2011) Liposome technology for industrial purposes. J Drug Deliv. doi:10.1155/2011/591325

    Google Scholar 

  41. Xia F, Hu D, Jin H, Zhao Y, Liang J (2012) Preparation of lutein proliposomes by supercritical anti-solvent technique. Food Hydrocoll 26:456–463

    Article  CAS  Google Scholar 

  42. Yoshida PA, Yokota D, Foglio MA, Rodrigues RAF, Pinho SC (2010) Liposomes incorporating essential oil of Brazilian cherry (Eugenia uniflora L.): characterization of aqueous dispersions and lyophilized formulations. J Microencapsul 27:416–425

    Article  CAS  Google Scholar 

  43. Zhao P, Xiong H, Peng H, Wang Q, Han D, Bai C, Li Y, Shi S, Deng B (2011) PEG-coated lyophilized proliposomes: preparation, characterization and in vitro release evaluation of vitamin E. Eur Food Res Technol 232:467–654

    Article  Google Scholar 

  44. Zou L-Q, Liu W, Liu W-L, Liang R-H, Li T, Liu C-M, Cao Y-L, Niu J, Liu Z (2014) Characterization and bioavailability of tea polyphenol nanoliposome prepared by combining an ethanol injection method with dynamic high-pressure microfluidization. J Agric Food Chem 62:934–941

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to FAPESP for the financial support (project 2009/01087-7) and the scholarships awarded (projects 2009/14868-7, 2011/01277-0, and 2011/03901-3). The authors also thank Danisco for the donation of xanthan gum and Carlos Alberto Paula Leite for the micrographs of the liposomes.

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Authors and Affiliations

  1. Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo (USP), São Paulo, Brazil

    José M. P. Carvalho, Taíse Toniazzo, Izabel C. F. Moraes & Samantha C. Pinho

  2. Brazilian Synchrotron Light National Laboratory, Campinas, Brazil

    Leide P. Cavalcanti

  3. Institute of Physics, University of São Paulo (USP), São Paulo, Brazil

    Cristiano L. P. Oliveira

  4. Department of Food Engineering, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte 225, Jd Elite, Pirassununga, SP, Brazil, 13635-900

    Samantha C. Pinho

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  1. José M. P. Carvalho
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  2. Taíse Toniazzo
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  3. Leide P. Cavalcanti
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  4. Izabel C. F. Moraes
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  5. Cristiano L. P. Oliveira
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  6. Samantha C. Pinho
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Correspondence to Samantha C. Pinho.

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Carvalho, J.M.P., Toniazzo, T., Cavalcanti, L.P. et al. Physico-chemical stability and structural characterization of thickened multilamellar beta-carotene-loaded liposome dispersions produced using a proliposome method. Colloid Polym Sci 293, 2171–2179 (2015). https://doi.org/10.1007/s00396-015-3594-8

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  • DOI: https://doi.org/10.1007/s00396-015-3594-8

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