Skip to main content

Advertisement

Log in

Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion

  • Original Paper
  • Published:
Histochemistry and Cell Biology Aims and scope Submit manuscript

Abstract

P2X receptors are ATP-gated cationic channels composed of seven cloned subunits (P2X1 –7). P2X3 homomultimer and P2X2/3 heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expression of the P2X5 receptor subunit in DRG in different species including mouse, rat, cat and guinea pig. Immunohistochemistry showed that P2X5 receptors exhibited low levels of immunostaining in rat DRG, but high levels in mouse and guinea pig. Only a few neurons were immunoreactive for P2X5 receptors in cat. In mouse DRG, the P2X5 receptor was expressed largely by medium-diameter neurons (42.9 %), less in small (29.3 %) and large (27.8 %) neurons. In contrast, in the guinea pig DRG, P2X5 receptor expression was greatest in small-diameter (42.6 %), less in medium- (36.3 %) and large-diameter (21.1 %) neurons. Colocalization experiments revealed that, in mouse DRG, 65.5, 10.9 and 27.1 % of P2X5 receptors were immunoreactive for NF-200, CGRP and calbindin, while only a few P2X5-immunoreactive (IR) neurons were coexpressed with IB4 or with NOS. In guinea pig DRG, a total of 60.5 and 40.5 % of P2X5-IR neurons were coexpressed with IB4 or with CGRP, while 20.3 and 24.5 % of P2X5 receptors were coexpressed with NF-200 or with NOS. Only a few P2X5-IR neurons were coexpressed with calbindin in guinea pig DRG. It will be of great interest to clarify the relative physiological and pathophysiological roles of P2X5 receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Boldogköi Z, Schütz B, Sallach J, Zimmer A (2002) P2X(3) receptor expression at early stage of mouse embryogenesis. Mech Dev 118:255–260

    Article  PubMed  Google Scholar 

  • Burnstock G (2006) Purinergic P2 receptors as targets for novel analgesics. Pharmacol Ther 110:433–454

    Article  PubMed  CAS  Google Scholar 

  • Burnstock G (2007) Physiology and pathophysiology of purinergic neurotransmission. Physiol Rev 87:659–797

    Article  PubMed  CAS  Google Scholar 

  • Burnstock G (2009) Purinergic receptors and pain. Curr Pharm Des 15:1717–1735

    Article  PubMed  CAS  Google Scholar 

  • Choi JS, Dib-Hajj SD, Waxman SG (2007) Differential slow inactivation and use-dependent inhibition of Nav1.8 channels contribute to distinct firing properties in IB4 + and IB4- DRG neurons. J Neurophysiol 97:1258–1265

    Article  PubMed  CAS  Google Scholar 

  • Collo G, North RA, Kawashima E, Merlo-Pich E, Neidhart S, Surprenant A, Buell G (1996) Cloning of P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. J Neurosci 16:2495–2507

    PubMed  CAS  Google Scholar 

  • Giniatullin R, Sokolova E, Nistri A (2003) Modulation of P2X3 receptors by Mg2 + on rat DRG neurons in culture. Neuropharmacology 44:132–140

    Article  PubMed  CAS  Google Scholar 

  • Glass R, Burnstock G (2001) Immunohistochemical identification of cells expressing ATP-gated cation channels (P2X receptors) in the adult rat thyroid. J Anat 198(Pt 5):569–579

    Article  PubMed  CAS  Google Scholar 

  • Guo W, Xu X, Gao X, Burnstock G, He C, Xiang Z (2008) Expression of P2X5 receptors in the mouse CNS. Neuroscience 156:673–692

    Article  PubMed  CAS  Google Scholar 

  • Illesa P, Ribeiro JA (2004) Molecular physiology of P2 receptors in the central nervous system. Eur J Pharmacol 483:5–17

    Article  Google Scholar 

  • James G, Butt AM (2002) P2Y and P2X purinoceptor mediated Ca2 + signalling in glial cell pathology in the central nervous system. Eur J Pharmacol 447:247–260

    Article  PubMed  CAS  Google Scholar 

  • Jarvis MF (2003) Contributions of P2X3 homomeric and heteromeric channels to acute and chronic pain. Expert Opin Ther Targets 7:513–522

    Article  PubMed  CAS  Google Scholar 

  • Kim C, Chung JM, Chung K (2003) Changes in the gene expression of six subtypes of P2X receptors in rat dorsal root ganglion after spinal nerve ligation. Neurosci Lett 337:81–84

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi K, Fukuoka T, Yamanaka H, Dai Y, Obata K, Tokunaga A, Noguchi K (2005) Differential expression patterns of mRNAs for P2X receptor subunits in neurochemically characterized dorsal root ganglion neurons in the rat. J Comp Neurol 481:377–390

    Article  PubMed  CAS  Google Scholar 

  • Li C (2000) Novel mechanism of inhibition by the P2 receptor antagonist PPADS of ATP-activated current in dorsal root ganglion neurons. J Neurophysiol 83:2533–2541

    PubMed  CAS  Google Scholar 

  • Luo ZD, Chaplan SR, Scott BP, Cizkova D, Calcutt NA, Yaksh TL (1999) Neuronal nitric oxide synthase mRNA upregulation in rat sensory neurons after spinal nerve ligation: lack of a role in allodynia development. J Neurosci 19:9201–9208

    PubMed  CAS  Google Scholar 

  • Pan AH, Lu DH, Luo XG, Chen L, Li ZY (2009) Formalin-induced increase in P2X(3) receptor expression in dorsal root ganglia: implications for nociception. Clin Exp Pharmacol Physiol 36:e6–e11

    Article  PubMed  CAS  Google Scholar 

  • Patil CS, Padi SV, Singh VP, Kulkarni SK (2006) Sildenafil induces hyperalgesia via activation of the NO-cGMP pathway in the rat neuropathic pain model. Inflammopharmacology 14:22–27

    Article  PubMed  CAS  Google Scholar 

  • Rose RD, Rohrlich D (1988) Counting sectioned cells via mathematical reconstruction. J Comp Neurol 272:365–386

    PubMed  CAS  Google Scholar 

  • Ruan HZ, Burnstock G (2003) Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat. Histochem Cell Biol 120:415–426

    Article  PubMed  CAS  Google Scholar 

  • Ruan HZ, Moules E, Burnstock G (2004) Changes in P2X3 purinoceptors in sensory ganglia of the mouse during embryonic and postnatal development. Histochem Cell Biol 122:539–551

    Article  PubMed  CAS  Google Scholar 

  • Ruan HZ, Birder LA, de Groat WC, Tai C, Roppolo J, Buffington CA, Burnstock G (2005) Localization of P2X and P2Y receptors in dorsal root ganglia of the cat. J Histochem Cytochem 53:1273–1282

    Article  PubMed  CAS  Google Scholar 

  • Ruan HZ, Birder LA, Xiang Z, Chopra B, Buffington T, Tai C, Roppolo JR, de Groat WC, Burnstock G (2006) Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder. Am J Physiol Renal Physiol 290:F1143–F1152

    Article  PubMed  CAS  Google Scholar 

  • Schädlich H, Wirkner K, Franke H, Bauer S, Grosche J, Burnstock G, Reichenbach A, Illes P, Allgaier C (2001) P2X(2), P2X(2–2) and P2X(5) receptor subunit expression and function in rat thoracolumbar sympathetic neurons. J Neurochem 79:997–1003

    Article  PubMed  Google Scholar 

  • Scherrer G, Imamachi N, Cao YQ, Contet C, Mennicken F, O’Donnell D, Kieffer BL, Basbaum AI (2009) Dissociation of the opioid receptor mechanisms that control mechanical and heat pain. Cell 137:1148–1159

    Article  PubMed  CAS  Google Scholar 

  • Sharp CJ, Reeve AJ, Collins SD, Martindale JC, Summerfield SG, Sargent BS, Bate ST, Chessell IP (2006) Investigation into the role of P2X(3)/P2X(2/3) receptors in neuropathic pain following chronic constriction injury in the rat: an electrophysiological study. Br J Pharmacol 148:845–852

    Article  PubMed  CAS  Google Scholar 

  • Tsuzuki K, Ase A, Séguéla P, Nakatsuka T, Wang CY, She JX, Gu JG (2003) TNP-ATP-resistant P2X ionic current on the central terminals and somata of rat primary sensory neurons. J Neurophysiol 89:3235–3242

    Article  PubMed  CAS  Google Scholar 

  • Ueno S, Tsuda M, Iwanaga T, Inoue K (1999) Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons. Br J Pharmacol 126:429–436

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Zhang X, Guo QL, Zou WY, Huang CS (2010) Yan JQ Cyclooxygenase inhibitors suppress the expression of P2X(3) receptors in the DRG and attenuate hyperalgesia following chronic constriction injury in rats. Neurosci Lett 478:77–81

    Article  PubMed  CAS  Google Scholar 

  • Wildman SS, Brown SG, Rahman M, Noel CA, Churchill L, Burnstock G, Unwin RJ, King BF (2002) Sensitization by extracellular Ca(2 +) of rat P2X(5) receptor and its pharmacological properties compared with rat P2X(1). Mol Pharmacol 62:957–966

    Article  PubMed  CAS  Google Scholar 

  • Xiang Z, He C, Burnstock G (2006) P2X5 receptors are expressed on neurons containing arginine vasopressin and nitric oxide synthase in the rat hypothalamus. Brain Res 1099:56–63

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 30672022 and 31000497).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Geoffrey Burnstock or Huai-Zhen Ruan.

Additional information

Jun-Wei Zeng and Sai-Yu Cheng contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zeng, JW., Cheng, SY., Liu, XH. et al. Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion. Histochem Cell Biol 139, 549–557 (2013). https://doi.org/10.1007/s00418-012-1046-9

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00418-012-1046-9

Keywords

Navigation