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Dissociation between force and maximal Na+, K+-ATPase activity in rat fast-twitch skeletal muscle with fatiguing in vitro stimulation

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Abstract

This study investigated whether high frequency in vitro stimulation of rat fast-twitch extensor digitorum longus muscle depresses Na+, K+-ATPase (NKA) activity as measured by the maximal in vitro 3-O-MFPase assay. EDL muscles subjected to 10 s continuous 100 Hz stimulation reduced tetanic force by 51.8 ± 5.1% which recovered to 81.2 ± 6.1% after 1 min and remained stable over 1 h recovery period. A second bout reduced force by 50.3 ± 3.8% of initial but had no effect on 3-O-MFPase activity. Three minutes of intermittent stimulation (1 s at 100 Hz and 4 s recovery) resulted in 87.0 ± 2.8% decline force with slow recovery (62.7 ± 5.8% of initial after 1 h). The second 3-min bout reduced force by 83.3 ± 3.6% of initial with no change in maximal 3-O-MFPase activity. These findings contrast previous human studies where fatiguing voluntary exercise depresses maximal NKA activity. This suggests that NKA in rat fast-twitch muscle is resistant to fatigue-induced inactivation under these conditions. Furthermore, the loss of force with fatigue was not related to depressed maximal NKA activity.

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Authors and Affiliations

  1. Muscle, Ions and Exercise Group, School of Human Movement, Recreation and Performance, Victoria University, Melbourne, VIC, Australia

    Craig A. Goodman & Michael J. McKenna

  2. School of Biomedical and Health Sciences, Victoria University, Melbourne, VIC, Australia

    Alan Hayes

  3. Centre for Ageing, Rehabilitation, Exercise and Sport, Victoria University, Melbourne, VIC, Australia

    Craig A. Goodman, Alan Hayes & Michael J. McKenna

  4. Department of Physiology, Faculty of Medicine, Dentistry and Health Science, The University of Melbourne, Parkville, VIC, 3010, Australia

    Craig A. Goodman

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  1. Craig A. Goodman
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Correspondence to Craig A. Goodman.

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Goodman, C.A., Hayes, A. & McKenna, M.J. Dissociation between force and maximal Na+, K+-ATPase activity in rat fast-twitch skeletal muscle with fatiguing in vitro stimulation. Eur J Appl Physiol 105, 575–583 (2009). https://doi.org/10.1007/s00421-008-0937-x

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