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E–C coupling and contractile characteristics of mechanically skinned single fibres from young rats during rapid growth and maturation

  • Skeletal Muscle
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Abstract

The postnatal growth of rats involves a developmental phase (0 to ∼3 weeks), a rapid growth phase (∼3 to ∼10 weeks), and a slower maturation phase (∼10 weeks+). In this study, we investigated the age-related changes in excitation–contraction (E–C) coupling characteristics of mammalian skeletal muscle, during rapid growth (4–10 weeks) and maturation (10–21 weeks) phases, using single, mechanically skinned fibres from rat extensor digitorum longus (EDL) muscle. Fibres from rats aged 4 and 8 weeks produced lower maximum T-system depolarization-induced force responses and fewer T-system depolarization-induced force responses to 75% run-down than those produced by fibres from rats aged 10 weeks and older. The sensitivity of the contractile apparatus to Ca2+ in fibres from 4-week rats was significantly higher than that in fibres from 10-week rats; however, the maximum Ca2+-activated force per skinned fibre cross-sectional area (specific force) developed by fibres from 4-week rats was on average ∼44% lower than the values obtained for all the other age groups. In agreement with the age difference in specific force, the MHC content of EDL muscles from 4-week rats was ∼29% lower than that of 10-week rats. Thus, mechanically skinned fibres from rats undergoing rapid growth are less responsive to T-system depolarization and maximal Ca2+ activation than fibres from rats at the later stage of maturation or adult rats. These results suggest that during the rapid growth phase in rats, the structure and function of elements involved in E–C coupling in fast-twitch skeletal muscle continue to undergo significant changes.

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Acknowledgements

This work was supported by the National Health and Medical Research Council (Australia) and the Australian Research Council.

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Authors and Affiliations

  1. School of Human Movement, Recreation and Performance, Centre for Ageing, Rehabilitation, Exercise and Sport, Victoria University, P.O. Box 14428, Melbourne, VIC, 8001, Australia

    C. A. Goodman

  2. Muscle Cell Biochemistry Laboratory, School of Biomedical and Health Sciences, Victoria University, P.O. Box 14428, Melbourne, VIC, 8001, Australia

    C. A. Goodman, R. Blazev, J. Kemp & G. M. M. Stephenson

  3. Centre of Physical Activity Across the Lifespan, School of Exercise Science, Australian Catholic University, Melbourne, VIC, 3165, Australia

    J. Kemp

  4. Department of Physiology, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia

    C. A. Goodman

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  1. C. A. Goodman
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  2. R. Blazev
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  3. J. Kemp
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  4. G. M. M. Stephenson
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Correspondence to C. A. Goodman.

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Drs. Goodman and Blazev contributed equally to the work presented in, and the preparation of, this manuscript.

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Goodman, C.A., Blazev, R., Kemp, J. et al. E–C coupling and contractile characteristics of mechanically skinned single fibres from young rats during rapid growth and maturation. Pflugers Arch - Eur J Physiol 456, 1217–1228 (2008). https://doi.org/10.1007/s00424-008-0474-9

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  • DOI: https://doi.org/10.1007/s00424-008-0474-9

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