Abstract
A family of Golgi-localised molecules was recently described in animals and fungi possessing extensive coiled regions and a short (~40 residues) conserved C-terminal domain, called the GRIP domain, which is responsible for their location to this organelle. Using the model plant Arabidopsis thaliana, we identified a gene (AtGRIP) encoding a putative GRIP protein. We demonstrated that the C-terminal domain from AtGRIP functions as a Golgi-targeting sequence in plant cells. Localisation studies in living cells expressing the AtGRIP fused to a DsRed2 fluorescent probe, showed extensive co-location with the Golgi marker α-mannosidase I in transformed tobacco protoplasts. GRIP-like sequences were also found in genomic databases of rice, maize, wheat and alfalfa, suggesting that this domain may be a useful Golgi marker for immunolocalisation studies. Despite low sequence identity amongst GRIP domains, the plant GRIP sequence was able to target to the Golgi of mammalian cells. Taken together, these data indicate that GRIP domain proteins might be implicated in a targeting mechanism that is conserved amongst eukaryotes.
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Abbreviations
- α-ManI:
-
α-Mannosidase I
- AtGRIP:
-
Arabidopsis GRIP domain protein
- GCC:
-
Golgi-localized coiled-coil protein
- GFP:
-
Green fluorescent protein
- MES:
-
2-(N-Morpholino)ethanesulfonic acid
- TGN:
-
Trans-Golgi network
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Acknowledgements
Paul R. Gilson and Claudia E. Vergara contributed equally to this work. We acknowledge the financial support of the Australian Research Council. We thank A.-C. Fitchette, Université de Rouen, for providing us with the anti-Lewis antigen antibody and thank Ross Waller and Tim Spurck, University of Melbourne, for assistance with confocal microscopy. We also thank Andrew Staehelin, University of Colorado, Boulder, for providing us with the α-Mannosidase I-GFP clone, Inhwan Hwang, Pohang University of Science and Technology, for the H+ATPase-GFP clone and Michael Goodin, University of California, Berkeley, for providing us with the pGD vectors.
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Gilson, P.R., Vergara, C.E., Kjer-Nielsen, L. et al. Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana. Planta 219, 1050–1056 (2004). https://doi.org/10.1007/s00425-004-1311-9
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DOI: https://doi.org/10.1007/s00425-004-1311-9