Abstract
In this study, cDNAs encoding myosin from the parasitic nematode Haemonchus contortus were isolated and characterized. Several exhibited a considerable degree of sequence variation at the nucleotide and limited divergence at the amino acid levels within the various functional domains. The results suggest that the cDNAs isolated represented a single myosin heavy chain, which, by comparison with a number of other myosins, is inferred to represent a homologue of a muscle myosin (CeMHCA) of the free-living nematode Caenorhabditis elegans. The findings could have implications for investigating cytoskeletal dynamics and/or signalling pathways.
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Acknowledgements
The authors would like to thank Michael Georgesz for the propagation of Caenorhabditis elegans. This work was supported by Novartis Animal Health Australasia Pty. Ltd., the Department of Primary Industries (DPI), Victoria, Australia, and the Australian Research Council (LP0346983 and LP0667795 to RBG). SN was the recipient of a postgraduate scholarship from the University of Melbourne and a “top-up award” from Novartis Animal Health.
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Nucleotide sequence data reported in this paper are available in the GenBank, EMBL and DDBJ databases under the accession nos. DQ310759–DQ310761.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-006-0188-x
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Hu, M., Chilton, N.B., Hartman, D. et al. Isolation and characterization of class II myosin genes from Haemonchus contortus . Parasitol Res 99, 200–203 (2006). https://doi.org/10.1007/s00436-006-0129-8
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DOI: https://doi.org/10.1007/s00436-006-0129-8