Abstract
Trichomonas vaginalis is a common sexually transmitted parasite in humans. Metronidazole has been the gold standard for treatment of trichomoniasis. The prevalence of metronidazole resistance and its unpleasant adverse effects drew the attention to the investigation of other lines of treatment, as that of herbal medicine. Garlic has been proven to have antibacterial, antiprotozoal, and antihelminthic activity. The current study was carried out to evaluate the efficacy of commercially available garlic (Tomex®) on T. vaginalis in vitro. The effect of different concentrations of garlic (12.5, 25, 50, and 100 μg/ml) was determined on multiplication and motility of trophozoites at different time points (after 24, 48, 72, and 96 h) in comparison to the same concentrations of metronidazole at the same different time points. The results showed that parasite multiplication inhibition was noticed in proportion of concentration of Tomex and incubation time. The minimal lethal concentration of Tomex was 100 μg/ml after 24 h, 50 μg/ml after 48 h, 25 μg/ml after 72 h, and 12.5 μg/ml after 96 h. These results were similar to that of metronidazole as its minimal lethal concentration was 50 μg/ml after 24 and 48 h and 12.5 μg/ml after 72 and 96 h. Garlic had completely inhibited the motility of trophozoites with concentration of 100 μg/ml after 24 h, 50 μg/ml after 48 h, 25 μg/ml after 72 h, and 12.5 μg/ml after 96 h while metronidazole had completely inhibited the motility of trophozoites with concentration of 50 μg/ml after 24 h, 25 μg/ml after 48 h, and 12.5 μg/ml after 72 and 96 h. This suggests that commercially available garlic (Tomex®) may be a promising phytotherapeutic agent for trichomoniasis.
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References
Abdel-Ghaffar F, Semmler M, Al-Rasheid KA, Strassen B, Fischer K, Aksu G, Klimpel S, Mehlhorn H (2011) The effects of different plant extracts on intestinal cestodes and on trematodes. Parasitol Res 108(4):979–984
Abdel-Hamid MY, Tawfeek GM, Cousha HS (1999) Efficacy of propolis (a bee product) against Hymenolepis nana infection, at different developmental stages, in immunocompetent and immunocompromised experimentally infected mice. Egypt J Med Sci 20(1):233–250
Afzan MY, Suresh K (2012) Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia. Parasitol Res 111(1):371–381
Ali NM (2007) In vitro activity of commercially available Egyptian propolis on Trichomonas vaginalis. The New Egyp J Med 36:7–15
Ankri S, Mirelman D (1999) Antimicrobial properties of allicin from garlic. Microbes Infect 1(2):125–129
Ankri S, Miron T, Rabinkov A, Wilchek M, Mirelman D (1997) Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob Agents Chemother 41(10):2286–2288
Bany J, Zdanowska D, Zdanowski R, Skopińska-Rózewska E (2003) The effect of herbal remedy on the development of Trichinella spiralis infection in mice. Pol J Vet Sci 6(3 Suppl):6–8
Blaha C, Duchêne M, Aspöck H, Walochnik J (2006) In vitro activity of hexadecylphosphocholine (miltefosine) against metronidazole-resistant and -susceptible strains of Trichomonas vaginalis. J Antimicrob Chemother 57:273–278
Conrad MD, Bradic M, Warring SD, Gorman AW, Carlton JM (2012) Getting trichy: tools and approaches to interrogating Trichomonas vaginalis in a post-genome world. Trends Parasitol 29(1):17–25
Coppi A, Cabinian M, Mirelman D, Sinnis P (2006) Antimalarial activity of allicin, a biologically active compound from garlic cloves. Antimicrob Agents Chemother 50(5):1731–1737
Davis SR (2005) An overview of the antifungal properties of allicin and its breakdown products—the possibility of a safe and effective antifungal prophylactic. Mycoses 48(2):95–100
Diamond LS (1957) The establishment of various trichomonds of animals and man in axenic cultures. J Parasitol 43:488–490
El Shenawy NS, Soliman MF, Reyad SI (2008) The effect of antioxidant properties of aqueous garlic extract and Nigella sativa as anti-schistosomiasis agents in mice. Rev Inst Med Trop Sao Paulo 50(1):29–36
Fani MM, Kohanteb J, Dayaghi M (2007) Inhibitory activity of garlic (Allium sativum) extract on multidrug-resistant Streptococcus mutans. J Indian Soc Pedod Prev Dent 25(4):164–168
Gradoni L, Ascenzi P (2004) Nitric oxide and anti-protozoan chemotherapy. Parasitologia 46(1–2):101–103
Harris JC, Plummer S, Turner MP, Lloyd D (2000) The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology 146(Pt 12):3119–3127
Kapoor K, Chandra M, Nag D, Paliwal JK, Gupta RC, Saxena RC (1999) Evaluation of metronidazole toxicity: a prospective study. Int J Clin Pharmacol Res 19:83–88
Lun ZR, Burri C, Menzinger M, Kaminsky R (1994) Antiparasitic activity of diallyl trisulphide (Dasuansu) on human and animal pathogenic protozoa (Trypanosoma sp., Entamoeba histolytica and Giardia lamblia) in vitro. Ann Soc Belge Med Trop 74:51–59
Mantawy MM, Mahmoud AH (2002) Effect of Allium cepa and Allium sativum feeding on glucose, glycogen, protein bands profile and phenol oxidase activity in Biomphalaria alexandrina. J Egypt Soc Parasitol 32(1):271–283
Meingassner JG, Thurner J (1979) Strain of Trichomonas vaginalis resistant to metronidazole and other 5-nitroimidazoles. Antimicrob Agents Chemother 15(2):254–257
Mirelman D, Monheit D, Varon S (1987) Inhibition of growth of Entamoeba histolytica by allicin, the active principle of garlic extract (Allium sativum). J Infect Dis 156:143–144
Nok AJ, Williams S, Onyenekwe PC (1996) Allium sativum-induced death of African trypanosomes. Parasitol Res 82(7):634–637
Palmas C, Wakelin D, Gabriele F (1984) Transfer of immunity against Hymenolepis nana in mice with lymphoid cells or serum from infected donors. Parasitology 89(Pt 2):287–293
Polat ZA, Vural A, Ozan F, Tepe B, Ozcelik S, Cetin A (2008) In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocul Pharmacol Ther 24(1):8–14
Rabiee S, Bazmani A, Matini M, Fallah M (2012) Comparison of resistant and susceptible strains of Trichomonas vaginalis to metronidazole using PCR method. Iran J Parasitol 7(3):24–30
Roberts J, Janovy J (2005) Foundation of parasitology, 7th edn. McGraw-Hill, New Jersey, pp 95–98
Rosolson M, Vanacova S, Tomkova E, Rezga J, Hardy IJ, Kulda J (2002) Mechanisms of in vitro development of resistance to metronidazole in Trichomonas vaginalis. Microbiology 148:2467–2477
Ross ZM, O'Gara EA, Hill DJ, Sleightholme HV, Maslin DJ (2001) Antimicrobial properties of garlic oil against human enteric bacteria: evaluation of methodologies and comparisons with garlic oil sulfides and garlic powder. Appl Environ Microbiol 67:475–480
Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR (2008) In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg 21(6):318–322
Schwebke JR (2002) Update of trichomoniasis. Sex Transm Infect 78:378–379
Shafir SC, Sorvillo FJ, Smith L (2009) Current issues and considerations regarding trichomoniasis and human immunodeficiency virus in African-Americans. Clin Microbiol Rev 22(1):37–45
Soffar SA, Mokhtar GM (1991) Evaluation of the antiparasitic effects of aqueous garlic (Allium sativum) extract in hymenolepiasis nana and giardiasis. J Egypt Soc Parasitol 21:497–502
WHO (1995) Metronidazole. In: Drugs used in parasitic diseases. WHO, Geneva
Yakoob J, Abbas Z, Beg MA, Naz S, Awan S, Hamid S, Jafri W (2011) In vitro sensitivity of Blastocystis hominis to garlic, ginger, white cumin, and black pepper used in diet. Parasitol Res 109(2):379–385
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Ibrahim, A.N. Comparison of in vitro activity of metronidazole and garlic-based product (Tomex®) on Trichomonas vaginalis . Parasitol Res 112, 2063–2067 (2013). https://doi.org/10.1007/s00436-013-3367-6
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DOI: https://doi.org/10.1007/s00436-013-3367-6