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Morphological and phylogenetic analysis of a microsporidium (Nosema sp.) isolated from rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae)

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Abstract

A new microsporidium was isolated from Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), one of the most important rice pests in China. The morphology and molecular systematics of this novel microsporidium were described in this study. The spores were long oval and measured 3.17 × 1.64 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: a diplokaryon, 10–12 polar filament coils of the same type, and posterior vacuole. Small subunit rRNA gene sequence data and phylogenetic analysis further confirmed that the microsporidian species from C. suppressalis belong to the true Nosema sub-group of the genus Nosema. Besides, the microsporidium Nosema sp. CS could cause systemic infection of Bombyx mori and infect silkworms through vertical transmission. Therefore, mulberry field pest control should be carefully monitored, and sanitation of mulberry leaves is essential to control the pebrine disease in sericulture.

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Acknowledgments

We thank all the authors for their free-charged software cited and used in this article. We are grateful to Lu Mingming and all our partners for their kind help. This work was supported by the Science and Technology Project of Guangdong Province under Grant No. 2015A040404032 and Open Project of State Key Laboratory of Silkworm Genome Biology under Grant No. 2120130332.

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Authors and Affiliations

  1. Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, No. 133 Dongguanzhuang Yiheng Road, Guangzhou, 510610, China

    Dongxu Xing, Qiong Yang, Sentai Liao, Qingrong Li, Chaoyi Zhao, Yang Xiao & Mingqiang Ye

  2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Lanzhi Han

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  1. Dongxu Xing
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  2. Qiong Yang
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  3. Sentai Liao
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  4. Lanzhi Han
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  7. Yang Xiao
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Correspondence to Qiong Yang.

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Xing, D., Yang, Q., Liao, S. et al. Morphological and phylogenetic analysis of a microsporidium (Nosema sp.) isolated from rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae). Parasitol Res 116, 2741–2746 (2017). https://doi.org/10.1007/s00436-017-5584-x

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  • DOI: https://doi.org/10.1007/s00436-017-5584-x

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