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Plasticity in developing rat uterine sensory nerves: the role of NGF and TrkA

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Abstract

In the present study we investigated the effects of infantile/prepubertal chronic oestrogen treatment, chemical sympathectomy with guanethidine and combined sympathectomy and chronic oestrogen treatment on developing sensory nerves of the rat uterus. Changes in sensory innervation were assessed quantitatively on uterine cryostat tissue sections stained for calcitonin gene-related peptide (CGRP). Uterine levels of NGF protein, using immunohistochemistry and ELISA, and mRNA, using Northern blots and in situ hybridization, were also measured. Finally, levels of TrkA NGF receptor in sensory neurons of T13 and L1 dorsal root ganglia (DRG), which supply the uterus, were assessed using densitometric immunohistochemistry. These studies showed that: (1) chronic oestrogen treatment led to an 83% reduction in the intercept density of CGRP-immunoreactive nerves; (2) sympathectomy had no effect on the density of uterine sensory nerves or on the pattern of oestrogen-induced changes; (3) NGF mRNA and protein increased following sympathectomy or chronic oestrogen treatment; and (4) oestrogen produced increased intensity of labelling (28%) for TrkA receptors in small-diameter sensory neurons, but decreased labelling (13%) in medium-sized neurons, which represent the large majority of the DRG neurons supplying the upper part of the uterine horn. Contrary to expectations, increased levels of NGF after sympathectomy and oestrogen treatment did not lead to increased sensory innervation of the uterus. The possibility that alterations in neuronal levels of TrkA contribute to the lack of response of uterine sensory nerves to the oestrogen-induced increase in NGF levels is discussed.

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Acknowledgements

The authors gratefully acknowledge the generous donation of NGF antibody by Dr. J.M. Conner. The technical assistance of Mrs. M.M. Portela, K. Shockley and N. Cuña is greatly appreciated.

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Authors and Affiliations

  1. Sección Bioquímica, Facultad de Ciencias, Iguá 4225, 11400, Montevideo, Uruguay

    C. Chalar

  2. Laboratorio de Biología Celular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, 11600, Montevideo, Uruguay

    A. Richeri, L. Viettro, R. Chávez-Genaro, P. Bianchimano, N. M. Marmol & M. M. Brauer

  3. Department of Neurosurgery, University of Cincinnati Medical School, Cincinnati, Ohio, USA

    K. Crutcher

  4. Autonomic Neuroscience Institute, Royal Free and University College Medical School, London, UK

    G. Burnstock

  5. Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, London, UK

    T. Cowen

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  1. C. Chalar
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Correspondence to M. M. Brauer.

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This work was supported by The Wellcome Trust, UK (CRIG Grant 058122/Z/99/Z/JC/KO), and PEDECIBA, Universidad de la República, Montevideo, Uruguay

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Chalar, C., Richeri, A., Viettro, L. et al. Plasticity in developing rat uterine sensory nerves: the role of NGF and TrkA. Cell Tissue Res 314, 191–205 (2003). https://doi.org/10.1007/s00441-003-0799-9

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