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Neutrophil depletion retards endometrial repair in a mouse model

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Abstract

The contribution of the high abundance of inflammatory cells present in the human endometrium prior to and during menstruation is unknown with respect to endometrial repair and/or menstruation. In this study, the presence and localisation of markers for key inflammatory cells have been examined in a mouse model of endometrial breakdown and repair and the functional contribution of neutrophils has been determined. In the model, decidualisation is artificially induced and progesterone support withdrawn; the endometrial tissue progressively breaks down by 24 h after progesterone withdrawal and, by 48 h, has usually undergone complete repair. Neutrophils have been identified in low abundance in decidual tissue, rise in number during breakdown and are most abundant during early repair. Macrophages are barely detectable during breakdown or repair in this model, whereas uterine natural killer cells are found only in intact decidua. The functional contribution of neutrophils to endometrial breakdown and repair has been assessed via neutrophil depletion by using the antibody RB6-8C5. This antibody significantly depletes neutrophils from the circulation and tissue, affects endometrial breakdown and markedly delays endometrial repair. This study has therefore demonstrated that neutrophils are the most abundant leucocyte in this model and that they play an important functional role in the processes of endometrial breakdown and repair.

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Acknowledgements

We thank Dr. Aidan Sudbury from the School of Mathematical Sciences, Monash University, for statistical assistance.

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Authors and Affiliations

  1. Level 4, Block E, Monash Medical Centre, Prince Henry’s Institute of Medical Research, 246 Clayton Road, Clayton, Victoria, 3168, Australia

    Tu’uhevaha J. Kaitu’u-Lino

  2. Prince Henry’s Institute of Medical Research, P.O. Box 5152, Clayton, Victoria, Australia

    Tu’uhevaha J. Kaitu’u-Lino, Naomi B. Morison & Lois A. Salamonsen

  3. Department of Obstetrics and Gynecology, Monash University, Clayton, Victoria, 3168, Australia

    Tu’uhevaha J. Kaitu’u-Lino

Authors
  1. Tu’uhevaha J. Kaitu’u-Lino
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  2. Naomi B. Morison
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  3. Lois A. Salamonsen
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Corresponding author

Correspondence to Tu’uhevaha J. Kaitu’u-Lino.

Additional information

This work was funded by the National Health and Medical Research Council of Australia (#143798, #241000) and by an Australian Postgraduate Scholarship to T.K.

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Kaitu’u-Lino, T.J., Morison, N.B. & Salamonsen, L.A. Neutrophil depletion retards endometrial repair in a mouse model. Cell Tissue Res 328, 197–206 (2007). https://doi.org/10.1007/s00441-006-0358-2

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  • DOI: https://doi.org/10.1007/s00441-006-0358-2

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