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Integrin β3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia

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Abstract

Streptococcus pneumoniae is one of the most common causes of bacterial pneumonias in humans. Neutrophil migration into lungs infected with S. pneumoniae is central to the host defense but the mechanisms of neutrophil recruitment, as mediated by S. pneumoniae, into lungs are incompletely understood. Therefore, we have assessed the role of integrin αvβ3 by evaluating its subunit β3 in a mouse model of lung inflammation induced by S. pneumonia. Integrin subunit β3 knockout (β3-/-) and wild-type (WT) mice were intratracheally instilled with either S. pneumoniae or saline. Other groups of WT mice were treated intraperitoneally with 25 μg or 50 μg of antibody against integrin β3 or with isotype-matched antibody at 1 h before instillation of S. pneumoniae. Mice were killed 24 h after infection. Flow cytometry confirmed the absence or presence of integrin subunit β3 on peripheral blood neutrophils in β3-/- or WT mice, respectively. Neutrophil numbers in bronchoalveolar lavage (BAL) from infected β3-/- and WT mice showed no differences. Neutrophil numbers in BAL of infected WT mice treated with β3 antibody were lower compared with those without antibody but similar to those of mice administered isotype-matched antibody. Many neutrophils were present in the perivascular spaces of the lungs in β3-/- mice. Lungs from infected β3-/- mice had negligible mitogen-activated protein kinase expression compared with those of infected WT mice. Thus, integrin β3 or its heterodimer αvβ3 is not critical for neutrophil migration into lungs infected with S. pneumoniae.

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Acknowledgements

We thank Ms. Andrea Aebischer for her assistance during the experiments. We are grateful to Mr. Philip Dillman, Prairie Diagnostic Services, for helping us with Gram’s staining of lung tissues and to Ms. Cathy Coghlin for aid with the flow cytometry.

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Correspondence to Baljit Singh.

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The study was funded through a grant from the Natural Sciences and Engineering Research Council of Canada to Dr. B. Singh. Dr. Janardhan was a recipient of a Graduate Merit Scholarship from the University of Saskatchewan and an Interprovincial Graduate Student Fellowship from the Western College of Veterinary Medicine. Dr. Charavaryamath was a recipient of a PHARE scholarship from CIHR.

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Janardhan, K.S., Charavaryamath, C., Aulakh, G.K. et al. Integrin β3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia. Cell Tissue Res 348, 177–187 (2012). https://doi.org/10.1007/s00441-011-1300-9

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