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Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells

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Abstract

A sub-group of enteroendocrine cells (L cells) release gastrointestinal hormones, GLP-1 and PYY, which have different but overlapping physiological effects, in response to intraluminal nutrients. Whilst their release profiles are not identical, how the plasma levels of these two hormones are differentially regulated is not well understood. We investigate the possibility that GLP-1 and PYY are in separate storage vesicles. In this study, the subcellular location of GLP-1 and PYY storage organelles is investigated using double-labelling immunohistochemistry, super resolution microscopy and high-resolution confocal microscopy. In all species tested, human, pig, rat and mouse, most cytoplasmic stores that exhibited GLP-1 or PYY immunofluorescence were distinct from each other. The volume occupancy, determined by 3D analysis, overlapped by only about 10∼20 %. At the lower resolution achieved by conventional confocal microscopy, there was also evidence of GLP-1 and PYY being in separate storage compartments but, in subcellular regions where there were many storage vesicles, separate storage could not be resolved. The results indicate that different storage vesicles in L cells contain predominantly GLP-1 or predominantly PYY. Whether GLP-1 and PYY storage vesicles are selectively mobilised and their products are selectively released needs to be determined.

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Acknowledgments

This work was supported by funding from Pancosma, SA. We thank Professor Frank Dunshea and Maree Cox of the Melbourne School of Land and Environment for supplying intestinal samples from pigs. 3D-SIM imaging was performed at the Biological Optical Microscopy Platform, The University of Melbourne (www.microscopy.unimelb.edu.au).

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Authors and Affiliations

  1. Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia

    Hyun-Jung Cho, Eliza S. Robinson, Leni R. Rivera & John B. Furness

  2. The Biological Optical Microscopy Platform, University of Melbourne, Parkville, Victoria, 3010, Australia

    Paul J. McMillan

  3. Department of Surgery, Austin Health, University of Melbourne, Heidelberg, Victoria, 3084, Australia

    Adam Testro & Mehrdad Nikfarjam

  4. Pancosma S.A., Geneva, 1218, Switzerland

    David M. Bravo

Authors
  1. Hyun-Jung Cho
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  2. Eliza S. Robinson
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  3. Leni R. Rivera
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  4. Paul J. McMillan
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  5. Adam Testro
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  8. John B. Furness
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Correspondence to John B. Furness.

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Cho, HJ., Robinson, E.S., Rivera, L.R. et al. Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells. Cell Tissue Res 357, 63–69 (2014). https://doi.org/10.1007/s00441-014-1886-9

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  • DOI: https://doi.org/10.1007/s00441-014-1886-9

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