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Regeneration of the digestive system in the crinoid Lamprometra palmata (Mariametridae, Comatulida)

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Abstract

The morphology and regeneration of the digestive system and tegmen after autotomy of the visceral mass in the crinoid Lamprometra palmata (Clark 1921) was studied. The gut has a five-lobed shape and is covered by a tegmen. The tegmen consists of epidermis and underlying connective tissue. The digestive tube can be divided into three parts: esophagus, intestine, and rectum. At 6 h post-autotomy, the calyx surface is covered by a layer of amoebocytes and juxtaligamental cells (JLCs). At 14–18 h, post-autotomy transdifferentiation of JLCs begins and give rise to the epidermis and cells of digestive system. On days 1–2 post-autotomy, JLCs undergo the mesenchymal–epithelial transition. Some JLCs turn into typical epidermal cells, while other JLCs form small closed epithelial structures that represent the gut anlage. On day 4 post-autotomy, the animals have a mouth opening and a small anal cone. On day 7 post-autotomy, the visceral mass and the digestive system become fully formed but are smaller than normal. A 24-h exposure of L. palmata individuals to a 10−7 M colchicine solution did not slow down regeneration, and the timing of gut formation was similar to that in the control animals. We conclude that JLCs are the major cell source for gut and epidermis regeneration in L. palmata. The main mechanisms of morphogenesis are cell migration, mesenchymal–epithelial transition, and transdifferentiation.

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Acknowledgments

The authors express their gratitude to Dr. Vo Si Tuan (Director of IO VAST) for the opportunity to work at IO VAST and to Mr. Hoang Trung Du for the assistance in collecting the material. Special thanks are due to Dr. E.S. Mekhova for the identification of feather star species.

Funding

This study was supported by the Ministry of Science and Higher Education of the Russian Federation (grant 13.1902.21.0012, contract no. 075-15-2020-796).

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  1. A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

    Kalacheva Nadezhda V., Kamenev Yaroslav O. & Dolmatov Igor Yu.

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V., K.N., O., K.Y. & Yu., D.I. Regeneration of the digestive system in the crinoid Lamprometra palmata (Mariametridae, Comatulida). Cell Tissue Res 391, 87–109 (2023). https://doi.org/10.1007/s00441-021-03526-4

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