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Influence of mass transfer limitations on the enzymatic synthesis of β-lactam antibiotics catalyzed by penicillin G acylase immobilized on glioxil-agarose

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Abstract

Mass transfer effects were investigated for the synthesis of ampicillin and amoxicillin, at pH 6.5 and 25 °C, catalyzed by penicillin G acylase immobilized on agarose. The influence of external mass transfer was analysed using different stirring rates, ranging form 200 to 800 rpm. Above 400 rpm, the film resistance may be neglected. Intra-particle diffusion limitation was investigated using biocatalysts prepared with different enzyme loads and agarose with different mean pore diameters. When agarose with 6, 8 and 10% of crosslinking were used, for the same enzyme load, substrates and products concentration profiles presented no expressive differences, suggesting pore diameter is not important parameter. An increase on enzyme load showed that when more than 90 IU of enzyme activity were used per mL of support, the system was influenced by intra-particle mass transfer. A reactive-diffusive model was used to estimate effective diffusivities of substrates and products.

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Acknowledgments

The authors would like to thank Brazilian research-funding agencies FAPESP (State of Sao Paulo), CNPq and CAPES (Federal), Hispanagar S.A. and Antibióticos S.A. for the agarose gel and the enzyme.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, CEP 60455-760, Fortaleza, CE, Brasil

    L. R. B. Gonçalves & A. L. O. Ferreira

  2. Instituto de Catálisis y Petroleoquímica, CSIC, Madrid, Spain

    R. Fernandez-Lafuente & J. M. Guisan

  3. Departamento de Engenharia Química, Universidade Federal de São Carlos, 13.565-905, C.P. 676, São Carlos, SP, Brasil

    R. C. Giordano & R. L. C. Giordano

Authors
  1. L. R. B. Gonçalves
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  2. A. L. O. Ferreira
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  3. R. Fernandez-Lafuente
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  4. J. M. Guisan
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  5. R. C. Giordano
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  6. R. L. C. Giordano
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Correspondence to L. R. B. Gonçalves.

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Gonçalves, L.R.B., Ferreira, A.L.O., Fernandez-Lafuente, R. et al. Influence of mass transfer limitations on the enzymatic synthesis of β-lactam antibiotics catalyzed by penicillin G acylase immobilized on glioxil-agarose. Bioprocess Biosyst Eng 31, 411–418 (2008). https://doi.org/10.1007/s00449-007-0176-2

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  • DOI: https://doi.org/10.1007/s00449-007-0176-2

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