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Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux

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Abstract

A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH2PO4, MgCl2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH2PO4 22.1 g/L; MgCl2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.

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Acknowledgments

This work was supported by a grant from the National Outstanding Youth Foundation of China (Grant No.: 21025625), the Major Basic R&D Program of China (2007CB707803), National Key Technology R&D Program (2008BAI63B07) and Natural Science Foundation of Jiangsu Province (BK2007527).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, People’s Republic of China

    Dong Liu, Yong Chen, An Li, Jingjing Xie, Jian Xiong, Jianxin Bai, Xiaochun Chen, Huanqing Niu, Tao Zhou & Hanjie Ying

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  1. Dong Liu
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  2. Yong Chen
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  3. An Li
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  4. Jingjing Xie
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  5. Jian Xiong
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  6. Jianxin Bai
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  7. Xiaochun Chen
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  8. Huanqing Niu
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  9. Tao Zhou
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  10. Hanjie Ying
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Correspondence to Hanjie Ying.

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Liu, D., Chen, Y., Li, A. et al. Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux. Bioprocess Biosyst Eng 35, 729–737 (2012). https://doi.org/10.1007/s00449-011-0653-5

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  • DOI: https://doi.org/10.1007/s00449-011-0653-5

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