Abstract
Spa mineral waters are used for the treatment of chronic diseases’ symptoms. Anti-inflammatory, analgesic, anti-ageing and tissue repair effects have been attributed to them. This work seeks to improve knowledge about the effect of spa mineral waters on human cells. For this, human lung fibroblasts were treated with mineral waters from Ledesma, Paracuellos and Archena spas, three Spanish health resorts with different water chemical composition. A significant increase of cell proliferation together with an enhanced antioxidant capacity (reactive oxygen and nitrogen species, glutathione levels and superoxide dismutase activity) in mineral water-treated fibroblasts compared to control fibroblasts was observed. Moreover, cytokine profiling revealed an increase in the release of MIF, IL-6, CL-1, CCL-5 and ICAM-1, which are described as mediators in proliferation, wound healing and cell migration processes. In conclusion, our results could be in line with the effects attributed to spa mineral waters in wound healing strategies and oxidative damage protection.
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Acknowledgements
The sponsor had no role in study design, data collection, data analysis, data interpretation, writing of the paper and in the decision to submit the article for publication. We are grateful to the colleagues of the Proteomics laboratory from “12 de Octubre” Hospital (Madrid) for the technical help provided. We are also grateful to the health resorts of Baños de Ledesma, Archena and Paracuellos de Jiloca for the technical assistance while sampling.
Funding
This work was supported by the UCLM research group “Agua y Salud” [grant numbers GI20163517 and GI20174056].
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LMMS, MTAO and IMG conceived and designed the study; LMMS, IGR and VPM performed the experiments; LMMS and MTAO analysed the data; LMMS, MTAO and IMG wrote the paper. All the authors revised the manuscript and approved the final version.
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Melgar-Sánchez, L.M., García-Ruiz, I., Pardo-Marqués, V. et al. Influence of mineral waters on in vitro proliferation, antioxidant response and cytokine production in a human lung fibroblasts cell line. Int J Biometeorol 63, 1171–1180 (2019). https://doi.org/10.1007/s00484-019-01730-0
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DOI: https://doi.org/10.1007/s00484-019-01730-0