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Metallomic study on plasma samples from Nile tilapia using SR-XRF and GFAAS after separation by 2D PAGE: initial results

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Abstract

An investigation was made on plasma samples obtained after protein separation. The proteome of the plasma of Nile tilapia (Oreochromis niloticus) was separated by 2D PAGE, and manganese and zinc in protein spots was qualitatively and quantitatively determined by synchrotron radiation X-ray fluorescence (SR-XRF) and graphite furnace atomic absorption spectrometry (GFAAS). Manganese and zinc are present in four and six plasma protein spots, respectively. These ions are bound to proteins with molecular weights ranging from 19 to 70 kDa and with isoelectric point (pI) ranging from 4.7 to 6.3. The concentrations of manganese and zinc bound to these proteins as determined by GFAAS following acid digestion of the spots range from 0.8 to 2.6 mg of manganese, and from 1.0 to 6.3 mg of zinc, respectively, per g of protein.

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Acknowledgments

The authors acknowledge the Brazilian institutions FAPESP (Processes 03/13362-6, 07/59778-0 and 08/55903-7) and CNPq (Process 301123/2005-5) for their financial support of this work.

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Authors and Affiliations

  1. Faculdade de Medicina Veterinária e Zootecnia/UNESP—Programa de Pós-Graduação em Zootecnia, C. Postal 560, 18618-970, Botucatu, SP, Brasil

    Felipe A. Santos, Paula M. Lima & Renato C. F. Neves

  2. Departamento de Química e Bioquímica, Instituto de Biociências/UNESP, C. Postal 510, 18618-970, Botucatu, SP, Brasil

    Paula M. Moraes, Gustavo R. Castro & Pedro de Magalhães Padilha

  3. Laboratório Nacional de Luz Síncrotron, C. Postal 6192, 13083-970, Campinas, SP, Brasil

    Carlos A. Pérez

  4. Instituto de Química/UNICAMP, Grupo de Espectrometria, Preparo de Amostras e Mecanização—GEPAM, C. Postal 6154, 13084-971, Campinas, SP, Brasil

    Marcelo O. A. Silva & Marco A. Z. Arruda

  5. Instituto Nacional de Ciência e Tecnologia de Bioanalítica/UNICAMP, C. Postal 6154, 13084-971, Campinas, SP, Brasil

    Marco A. Z. Arruda & Pedro de Magalhães Padilha

Authors
  1. Felipe A. Santos
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  2. Paula M. Lima
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  3. Renato C. F. Neves
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  4. Paula M. Moraes
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  5. Carlos A. Pérez
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  6. Marcelo O. A. Silva
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  7. Marco A. Z. Arruda
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  8. Gustavo R. Castro
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  9. Pedro de Magalhães Padilha
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Corresponding author

Correspondence to Pedro de Magalhães Padilha.

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Santos, F.A., Lima, P.M., Neves, R.C.F. et al. Metallomic study on plasma samples from Nile tilapia using SR-XRF and GFAAS after separation by 2D PAGE: initial results. Microchim Acta 173, 43–49 (2011). https://doi.org/10.1007/s00604-010-0522-y

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  • DOI: https://doi.org/10.1007/s00604-010-0522-y

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