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Stir bar sorptive extraction of propranolol from plasma samples using a steel pin coated with a polyaniline and multiwall carbon nanotube composite

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Abstract

We have developed a simple and sensitive method for the determination of propranolol in plasma samples. It is based on stir-bar sorptive extraction and HPLC with fluorescence detection. Extraction is accomplished with a nanocomposite consisting of polyaniline and multiwall carbon nanotubes that was electrochemically prepared on a steel pin. The extraction and desorption time, desorption solvent, ionic strength and pH value of sample matrix were optimized. The method has a linear (r = 0.9917) response to propranolol in the 0.1–15 ng mL−1 concentration range. Relative standard deviations of intra-day (n = 5) and inter-day (n = 3) assay precision are < 8.3 % and 10.4 %, respectively. The enrichment factor for plasma is 8.5, and the limit of detection is as low as 30 pg mL−1. Obviously, the use of nanotubes can largely improve the extraction efficiencies when compared to the performance of a plain polyaniline coating. The method was successfully applied to the determination of propranolol in spiked human plasma samples.

A stir bar based on polyaniline/carbon nanotubes was prepared and applied for the extraction and sensitive determination of propranolol in plasma by coupling with HPLC. The limit of detection was 30 pg mL−1 which are lower than those in published papers.

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Acknowledgment

The authors appreciate Mrs. Sima Farhadi for her efforts with the English edition of the text.

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Correspondence to Khalil Farhadi.

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Farhadi, K., Firuzi, M. & Hatami, M. Stir bar sorptive extraction of propranolol from plasma samples using a steel pin coated with a polyaniline and multiwall carbon nanotube composite. Microchim Acta 182, 323–330 (2015). https://doi.org/10.1007/s00604-014-1336-0

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  • DOI: https://doi.org/10.1007/s00604-014-1336-0

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