Skip to main content
SpringerLink
Log in

Aptamer-AuNP-conjugated carboxymethyl chitosan–functionalized graphene oxide for colorimetric identification of Salmonella typhimurium

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A novel aptamer-AuNP-conjugated carboxymethyl chitosan–functionalized graphene oxide (CMC/GO@Apt-Au NP) probe was for the first time developed for the determination of Salmonella typhimurium (S. typhimurium). Owing to the conformational change of the aptamers in the presence of S. typhimurium, the Au NPs, which were pre-adsorbed on the aptamers through van der Waals forces, were released into the solution phase and induced the color change of the solution. As a result, S. typhimurium ranging from 102 to 107 CFU/mL was successfully identified using the designed assay with a limit of detection (LOD) of 10 CFU/mL. This low detection level allowed the sensitive recognition of S. typhimurium in milk samples within 40 min without sample pretreatment, a conclusion that agreed well with the traditional plate counting method. The developed method not only provides a rapid way for the determination of S. typhimurium with simplicity and sensitivity but also shows potential universality in the quantification of other pathogenic microorganisms.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. O’Bryan CA, Ricke SC, Marcy JA (2022) Public health impact of Salmonella spp on raw poultry: current concepts and future prospects in the United States. Food Control 132:108539

    Article  Google Scholar 

  2. Wang Z, Xu Q, Liu S, Liu Y, Gao Y, Wang M, Zhang L, Chang H, Wei Q, Sui Z (2022) Rapid and multiplexed quantification of Salmonella, Escherichia coli O157:H7, and Shigella flexneri in ground beef using flow cytometry. Talanta 238:123005

    Article  CAS  PubMed  Google Scholar 

  3. Sannigrahi S, Arumugasamy SK, Mathiyarasu J, Suthindhiran K (2020) Magnetosome-anti-Salmonella antibody complex based biosensor for the detection of Salmonella typhimurium. Mater Sci Eng C 114:111071

    Article  CAS  Google Scholar 

  4. Pang T, Bhutta ZA, Finlay BB, Altwegg M (1995) Typhoid fever and other salmonellosis: a continuing challenge. Trends Microbiol 3:253–255

    Article  CAS  PubMed  Google Scholar 

  5. Shen Y, Xu L, Li Y (2021) Biosensors for rapid detection of Salmonella in food: a review. Compr Rev Food Sci Food Saf 20:149–197

    Article  PubMed  Google Scholar 

  6. Lin L, Zheng Q, Lin J, Yuk HG, Guo L (2020) Immuno- and nucleic acid-based current technique for Salmonella detection in food. Eur Food Res Technol 246:373–395

    Article  CAS  Google Scholar 

  7. Bayraç C, Eyidoğan F, AvniÖktem H (2017) DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis Biosens. Bioelectron 98:22–28

    Article  Google Scholar 

  8. Wu Y, Wu M, Liu C, Tian Y, Fang S, Yang H, Li B, Liu Q (2021) Colloidal gold immunochromatographic test strips for broad-spectrum detection of Salmonella. Food Control 126:108052

    Article  CAS  Google Scholar 

  9. Zheng L, Cai G, Qi W, Wang S, Wang M, Lin J (2020) Optical biosensor for rapid detection of Salmonella typhimurium based on porous gold@platinum nanocatalysts and a 3D fluidic chip. ACS Sens 5:65–72

    Article  CAS  PubMed  Google Scholar 

  10. Joshi DJ, Koduru JR, Malek NI, Hussain CM, Kailasa SK (2021) Surface modifications and analytical applications of graphene oxide: a review, TrAC. Trends Anal Chem 144:116448

    Article  CAS  Google Scholar 

  11. Gao L, Lian C, Zhou Y, Yan L, Li Q, Zhang C, Chen L, Chen K (2014) Graphene oxide-DNA based sensors. Biosens Bioelectron 60:22–29

    Article  CAS  PubMed  Google Scholar 

  12. Zhao XH, Kong RM, Zhang XB, Meng HM, Liu WN, Tan W, Shen GL, Yu RQ (2011) Graphene-DNAzyme based biosensor for amplified fluorescence “turn-on” detection of Pb2+ with a high selectivity. Anal Chem 83:5062–5066

    Article  CAS  PubMed  Google Scholar 

  13. Zhu Y, Cai Y, Xu L, Zheng L, Wang L, Qi B, Xu C (2015) Building An aptamer/graphene oxide FRET biosensor for one-step detection of bisphenol A. ACS Appl Mater Interfaces 7:7492–7496

    Article  CAS  PubMed  Google Scholar 

  14. Zhang Z, Xiang X, Hu Y, Deng Y, Li L, Zhao W, Wu T (2021) A sensitive biomolecules detection device with catalytic hairpin assembly and cationic conjugated polymer-assisted dual signal amplification strategy. Talanta 223:121716

    Article  CAS  PubMed  Google Scholar 

  15. Qin J, Cui X, Wu P, Jiang Z, Chen Y, Yang R, Hu Q, Sun Y, Zhao S (2017) Fluorescent sensor assay for β-lactamase in milk based on a combination of aptamer and graphene oxide. Food Control 73:726–733

    Article  CAS  Google Scholar 

  16. Weng X, Neethirajan S (2016) A microfluidic biosensor using graphene oxide and aptamer-functionalized quantum dots for peanut allergen detection. Biosens Bioelectron 85:649–656

    Article  CAS  PubMed  Google Scholar 

  17. Chinnappan R, AlAmer S, Eissa S, Rahamn AA, Abu Salah KM, Zourob M (2017) Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamer. Microchim. Acta 185:61

    Article  Google Scholar 

  18. Ma DL, He HZ, Leung KH, Zhong HJ, Chan DSH, Leung CH (2013) Label-free luminescent oligonucleotide-based probes. Chem Soc Rev 42:3427–3440

    Article  CAS  PubMed  Google Scholar 

  19. Wei H, Li B, Li J, Wang E, Dong S (2007) Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes. Chem Commun 36:3735–3737

    Article  Google Scholar 

  20. Li H, Rothberg L (2004) Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc Natl Acad Sci USA 101:14036–14039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Sun J, Lu Y, He L, Pang J, Yang F, Liu Y (2020) Colorimetric sensor array based on gold nanoparticles: design principles and recent advances, TrAC. Trends Anal Chem 122:115754

    Article  CAS  Google Scholar 

  22. Liu Z, Liu H, Wang L, Su X (2016) A label-free fluorescence biosensor for highly sensitive detection of lectin based on carboxymethyl chitosan-quantum dots and gold nanoparticles. Anal Chim Acta 932:88–97

    Article  CAS  PubMed  Google Scholar 

  23. Zimet P, Mombrú ÁW, Mombrú D, Castro A, Villanueva JP, Pardo H, Rufo C (2019) Physico-chemical and antilisterial properties of nisin-incorporated chitosan/carboxymethyl chitosan films. Carbohydr Polym 219:334–343

    Article  CAS  PubMed  Google Scholar 

  24. Shariatinia Z (2018) Carboxymethyl chitosan: properties and biomedical applications. Int J Biol Macromol 120:1406–1419

    Article  CAS  PubMed  Google Scholar 

  25. Tian L, Qi J, Ma X, Wang X, Yao C, Song W, Wang Y (2018) A facile DNA strand displacement reaction sensing strategy of electrochemical biosensor based on N-carboxymethyl chitosan/molybdenum carbide nanocomposite for microRNA-21 detection. Biosens Bioelectron 122:43–50

    Article  CAS  PubMed  Google Scholar 

  26. Zhang M, Yang M, Woo MW, Li Y, Han W, Dang X (2021) High-mechanical strength carboxymethyl chitosan-based hydrogel film for antibacterial wound dressing. Carbohydr Polym 256:117590

    Article  CAS  PubMed  Google Scholar 

  27. Yi J, Wu P, Li G, Xiao W, Li L, He Y, He Y, Ding P, Chen C (2019) A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium. Microchim Acta 186:711

    Article  Google Scholar 

  28. Al-Gaashani R, Najjar A, Zakaria Y, Mansour S, Atieh M (2019) XPS and structural studies of high quality graphene oxide and reduced graphene oxide prepared by different chemical oxidation methods. Ceram Int 45:14439–14448

    Article  CAS  Google Scholar 

  29. Yuan D, Cadien K, Liu Q, Zeng H (2019) Adsorption characteristics and mechanisms of O-carboxymethyl chitosan on chalcopyrite and molybdenite. J Colloid Interface Sci 552:659–670

    Article  CAS  PubMed  Google Scholar 

  30. Huang Z, Li Z, Zheng L, Zhou L, Chai Z, Wang X, Shi W (2017) Interaction mechanism of uranium (VI) with three-dimensional graphene oxide-chitosan composite: insights from batch experiments, IR, XPS, and EXAFS spectroscopy. Chem Eng J 328:1066–1074

    Article  CAS  Google Scholar 

  31. Du S, Wang Y, Liu Z, Xu Z, Zhang H (2019) A portable immune-thermometer assay based on the photothermal effect of graphene oxides for the rapid detection of Salmonella typhimurium. Biosens Bioelectron 144:111670

    Article  CAS  PubMed  Google Scholar 

  32. Srisa-Art M, Boehle KE, Geiss BJ, Henry CS (2018) Highly sensitive detection of Salmonella typhimurium using a colorimetric paper-based analytical device coupled with immunomagnetic separation. Anal Chem 90:1035–1043

    Article  CAS  PubMed  Google Scholar 

  33. Hu J, Jiang YZ, Tang M, Wu LL, Xie HY, Zhang ZL, Pang DW (2019) Colorimetric-fluorescent-magnetic nanosphere-based multimodal assay platform for Salmonella detection. Anal Chem 91:1178–1184

    Article  CAS  PubMed  Google Scholar 

  34. Wang X, Niazi S, Yukun H, Sun W, Wu S, Duan N, Hun X, Wang Z (2017) Homogeneous time-resolved FRET assay for the detection of Salmonella typhimurium using aptamer-modified NaYF4:Ce/Tb nanoparticles and a fluorescent DNA label. Microchim Acta 184:4021–4027

    Article  CAS  Google Scholar 

  35. Wang S, Zheng L, Cai G, Liu N, Liao M, Li Y, Zhang X, Lin J (2019) A microfluidic biosensor for online and sensitive detection of Salmonella typhimurium using fluorescence labeling and smartphone video processing. Biosens Bioelectron 140:111333

    Article  CAS  PubMed  Google Scholar 

  36. Guo R, Wang S, Huang F, Chen Q, Li Y, Liao M, Lin J (2019) Rapid detection of Salmonella Typhimurium using magnetic nanoparticle immunoseparation, nanocluster signal amplification and smartphone image analysis. Sens Actuators, B 284:134–139

    Article  CAS  Google Scholar 

  37. Hu J, Tang F, Wang L, Tang M, Jiang YZ, Liu C (2021) Nanozyme sensor based-on platinum-decorated polymer nanosphere for rapid and sensitive detection of Salmonella typhimurium with the naked eye. Sens Actuators, B 346:130560

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the National Natural Science Foundation of China (grant number 82073607), the Natural Science Foundation of Hunan Province (2021JJ30637), the start-up funds from Central South University (grant number 202045008), and the Fundamental Research Funds for the Central Universities of Central South University (grant number 2022ZZTS0893).

Author information

Author notes

  1. Pian Wu and Ruixue Huang contributed equally to this work

Authors and Affiliations

  1. Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China

    Pian Wu, Ruixue Huang, Tianhan Kai, Yujuan Zhan, Xiaoqian Wei, Danqi Wang, Jingwen Zhang & Ping Ding

  2. Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China

    Pian Wu, Ruixue Huang, Tianhan Kai, Yujuan Zhan, Xiaoqian Wei, Danqi Wang, Jingwen Zhang & Ping Ding

  3. School of Public Health, Xiang Nan University, Chenzhou, 423000, Hunan, China

    Cuimei Chen

  4. School of Public Health, Changsha Medical University, Changsha, 410219, Hunan, China

    Jiecan Yi

Authors
  1. Pian Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruixue Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cuimei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiecan Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tianhan Kai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yujuan Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiaoqian Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Danqi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jingwen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ping Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ping Ding.

Ethics declarations

Competing Interests

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 892 KB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, P., Huang, R., Chen, C. et al. Aptamer-AuNP-conjugated carboxymethyl chitosan–functionalized graphene oxide for colorimetric identification of Salmonella typhimurium. Microchim Acta 189, 408 (2022). https://doi.org/10.1007/s00604-022-05494-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-022-05494-0

Keywords

Search

Navigation