Abstract
Brachypodium distachyon is a widely recognized model plant belonging to subfamily Pooideae with a sequenced genome. To gain a better understanding of the male reproductive development in B. distachyon we examined pollen morphology and cytochemical changes of microspore cytoplasm from pollen mother cell stage to mature pollen using light, fluorescent and scanning electron microscopy. Our results show that B. distachyon exhibits a typical monocot-type pollen ontogeny. Meiosis in the pollen mother cells is accomplished by successive cytokinesis generating isobilateral tetrads. Cytochemical examination indicated that microspore cytoplasm contains variable amounts of insoluble carbohydrates and proteins at different developmental stages. Deposition of starch in the cytoplasm of microspores starts at the bicellular stage and continues till the mature pollen stage. The formation of the exine wall progresses by the deposition of sporopollenin from the tapetum layer of the anther. The mature pollen is trinucleate, spheroidal in shape and possesses a single pore with an annulus and operculum. The exine pattern is smooth and of granular type.
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Acknowledgments
We thank Dr. Simon Crawford for technical guidance and access to Advanced Microscopy Facility, The University of Melbourne. Author; AS, also thanks Dr. Martin O’Brien for giving valuable suggestions. Financial support from the Australian Research Council (ARC DPO988972) is also gratefully acknowledged.
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Sharma, A., Singh, M.B. & Bhalla, P.L. Cytochemistry of pollen development in Brachypodium distachyon . Plant Syst Evol 300, 1639–1648 (2014). https://doi.org/10.1007/s00606-014-0989-9
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DOI: https://doi.org/10.1007/s00606-014-0989-9