Summary
The endogenous compound hydroxylamine relaxes vascular smooth muscle in vitro, apparently through conversion to the vasodilator factor nitric oxide, but its effect on blood pressure has not been characterized. We found that in the anesthetized rat the amine elicits dose-related hypotension when administered by continuous iv infusion. In experiments designed to explore the mechanism of this effect, hydroxylamine was compared with the nitric oxide donor nitroprusside and the direct-acting vasodilator hydralazine, using pretreatments known to modify diverse mechanisms of vasodilation. Hydroxylamine hypotension was enhanced by the SSAO inhibitor isoniazid and the SSAO substrate methylamine, a pattern shared by hydralazine. Responses were blocked by the guanylate cyclase inhibitor methylene blue and were increased by the nitric oxide synthase inhibitor L-NAME, a pattern shared by nitroprusside. It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a “hydralazine-like” mechanism involving SSAO inhibition.
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References
J Gil-Longo C González-Vázquez (2005) ArticleTitleCharacterization of four different effects elicited by H2O2 in rat aorta Vascul Pharmacol 43 128–138 10.1016/j.vph.2005.06.001 Occurrence Handle10.1016/j.vph.2005.06.001 Occurrence Handle1:CAS:528:DC%2BD2MXmsVOntL0%3D Occurrence Handle15994130
P Gross (1985) ArticleTitleBiologic activity of hydroxylamine: a review Crit Rev Toxicol 14 87–99 Occurrence Handle10.3109/10408448509023765 Occurrence Handle1:CAS:528:DyaL2MXht1Klt7Y%3D Occurrence Handle3882333
M Klink A Swierzko Z Sulowska (2001) ArticleTitleNitric oxide generation from hydroxylamine in the presence of neutrophils and in the cell-free system APMIS 109 493–499 10.1111/j.1600-0463.2001.apm090702.x Occurrence Handle10.1111/j.1600-0463.2001.apm090702.x Occurrence Handle1:CAS:528:DC%2BD3MXns1KjsL4%3D Occurrence Handle11552946
R Kruszyna H Kruszyna PA Doherty RP Smith (1984) ArticleTitleHypotensive effects of hydroxylamine in intact anesthetized dogs and cats Arch Toxicol 55 203–205 10.1007/BF00316130 Occurrence Handle10.1007/BF00316130 Occurrence Handle1:CAS:528:DyaL2cXlvVGms78%3D Occurrence Handle6497654
R Lewinsohn KH Böhm V Glover M Sandler (1978) ArticleTitleA benzylamine oxidase distinct from monoamine oxidase B — widespread distribution in man and rat Biochem Pharmacol 27 1857–1863 10.1016/0006-2952(78)90033-3 Occurrence Handle10.1016/0006-2952(78)90033-3 Occurrence Handle1:CAS:528:DyaE1MXhtlGnt7g%3D Occurrence Handle708467
GA Lyles I Singh (1985) ArticleTitleVascular smooth muscle cells: a major source of the semicarbazide-sensitive amine oxidase of the rat aorta J Pharm Pharmacol 37 637–643 Occurrence Handle1:CAS:528:DyaL2MXlsFOqtrc%3D Occurrence Handle2867184
GA Lyles J García-Rodríguez BA Callingham (1983) ArticleTitleInhibitory actions of hydralazine upon monoamine oxidizing enzymes in the rat Biochem Pharmacol 32 2515–2521 10.1016/0006-2952(83)90012-6 Occurrence Handle10.1016/0006-2952(83)90012-6 Occurrence Handle1:CAS:528:DyaL3sXlvVOgsbo%3D Occurrence Handle6615547
S Moncada DD Rees R Schulz RMJ Palmer (1991) ArticleTitleDevelopment and mechanism of a specific supersensitivity to nitrovasodilators after inhibition of vascular nitric oxide synthesis in vivo Proc Natl Acad Sci USA 88 2166–2170 10.1073/pnas.88.6.2166 Occurrence Handle10.1073/pnas.88.6.2166 Occurrence Handle1:CAS:528:DyaK3MXhvVaku7o%3D Occurrence Handle1848694
RM Rapoport F Murad (1984) ArticleTitleEffect of cyanide on nitrovasodilator-induced relaxation, cyclic GMP accumulation and guanylate cyclase activation in rat aorta Eur J Pharmacol 104 61–70 10.1016/0014-2999(84)90369-8 Occurrence Handle10.1016/0014-2999(84)90369-8 Occurrence Handle1:CAS:528:DyaL2cXlvVGmsb8%3D Occurrence Handle6149944
J Taira V Misik P Riesz (1997) ArticleTitleNitric oxide formation from hydroxylamine by myoglobin and hydrogen peroxide Biochem Biophys Acta 1336 502–508 Occurrence Handle1:CAS:528:DyaK2sXmtFKht74%3D Occurrence Handle9367178
H Vidrio (2003) ArticleTitleSemicarbazide-sensitive amine oxidase: role in the vasculature and vasodilation after in situ inhibition Auton Autacoid Pharmacol 23 275–283 10.1111/j.1474-8673.2004.00296.x Occurrence Handle10.1111/j.1474-8673.2004.00296.x Occurrence Handle1:CAS:528:DC%2BD2cXmtFKrtr8%3D Occurrence Handle15255812
H Vidrio M Medina G Fernández M Lorenzana-Jiménez AE Campos (2002) ArticleTitleEnhancement of hydralazine hypotension by low doses of isoniazid. Possible role of semicarbazide-sensitive amine oxidase inhibition Gen Pharmacol 35 195–204
H Vidrio M Medina P González-Romo M Lorenzana-Jiménez P Díaz-Arista A Baeza (2003) ArticleTitleSemicarbazide-sensitive amine oxidase substrates potentiate hydralazine hypotension: possible role of hydrogen peroxide J Pharmacol Exp Ther 307 497–504 10.1124/jpet.103.055350 Occurrence Handle10.1124/jpet.103.055350 Occurrence Handle1:CAS:528:DC%2BD3sXos1SitLw%3D Occurrence Handle12970383
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Vidrio, H., Medina, M. Hypotensive effect of hydroxylamine, an endogenous nitric oxide donor and SSAO inhibitor. J Neural Transm 114, 863–865 (2007). https://doi.org/10.1007/s00702-007-0702-z
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DOI: https://doi.org/10.1007/s00702-007-0702-z