Summary.
The envelope glycoprotein 2 (gp2) of equine herpesvirus 1 (EHV-1) has no known homologue in other herpesviruses with the exception of some equid alphaherpesviruses. In order to investigate the potential of gp2 as a vaccine antigen, expression vectors were constructed to encode full-length gp2, a truncated version lacking the membrane anchor, and the C-terminal region. Intramuscular inoculation of mice with these DNA constructs induced neutralizing antibody against EHV-1 and, following intranasal challenge with EHV-1, mice inoculated with any of the gp2 DNA constructs cleared virus more rapidly from their lungs than control mice. The rate of clearance was comparable to that for glycoprotein D DNA, indicating gp2 as a potential antigen for inclusion in a subunit vaccine.
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Present address: Faculty of Veterinary Science, University of Melbourne, Vic 3010, Australia.
Received January 27, 2003; accepted April 29, 2003 Published online July 17, 2003
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Learmonth, G., Love, D., Gilkerson, J. et al. Inoculation with DNA encoding the glycoprotein gp2 reduces severity of equine herpesvirus 1 infection in a mouse respiratory model. Arch Virol 148, 1805–1813 (2003). https://doi.org/10.1007/s00705-003-0146-1
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DOI: https://doi.org/10.1007/s00705-003-0146-1