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Reinforced poliovirus and enterovirus surveillance in Romania, 2015–2016

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Abstract

Due to the risk of poliovirus importation from Ukraine in 2015, a combined surveillance program monitoring the circulation of enteroviruses (EVs) in healthy children from at-risk areas and in the environment was conducted in Romania. Virological testing of stool samples collected from 155 healthy children aged from two months to six years and of 186 sewage water samples collected from different areas was performed. A total of 58 (37.42%) stool samples and 50 (26.88%) sewage water samples were positive for non-polio EVs, but no poliovirus was detected. A high level of circulation of echovirus (E) types 6 and 7 and coxsackievirus (CV) type B5 was observed.

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References

  1. Bessaud M, Blondel B, Joffret ML, Mac Kain A, Delpeyroux F (2018) Nomenclature and classification of the enteroviruses: story of a long history. Virologie 22(6):289–303

    Article  PubMed  Google Scholar 

  2. Zell R, Delwart E, Gorbalenya AE, Hovi T, King AMQ, Knowles NJ et al (2017) ICTV virus taxonomy profile: picornaviridae. J Gen Virol 98(10):2421–2422

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kew O, Pallansch M (2018) Breaking the last chains of poliovirus transmission: progress and challenges in global polio eradication. Annu Rev Virol 5(1):427–451

    Article  CAS  PubMed  Google Scholar 

  4. Vashishtha VM, Kamath S (2016) A brief history of vaccines against polio. Indian Pediatr 53(Suppl 1):S20–S27

    PubMed  Google Scholar 

  5. Combelas N, Holmblat B, Joffret ML, Colbere-Garapin F, Delpeyroux F (2011) Recombination between poliovirus and coxsackie A viruses of species C: a model of viral genetic plasticity and emergence. Viruses 3(8):1460–1484

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Burns CC, Diop OM, Sutter RW, Kew OM (2014) Vaccine-derived polioviruses. J Infect Dis 1(210 Suppl 1):S283–S293

    Article  Google Scholar 

  7. Anonymous (2005) Certification of poliomyelitis eradication-certification of poliomyelitis eradication fifteenth meeting of the European Regional Certification Commission Copenhagen, 19–21 June 2002. Copenhagen: World Health Organization—Regional Office for Europe

  8. Holt E (2013) Ukraine at risk of polio outbreak. Lancet 381(9885):2244

    Article  PubMed  Google Scholar 

  9. Anonymous (2003) Guidelines for environmental surveillance of poliovirus circulation. In: Biologicals Va (ed). World Health Organization, Geneva

  10. Wood DJ, Hull B (1999) L20B cells simplify culture of polioviruses from clinical samples. J Med Virol 58(2):188–192

    Article  CAS  PubMed  Google Scholar 

  11. Joffret ML, Polston PM, Razafindratsimandresy R, Bessaud M, Heraud JM, Delpeyroux F (2018) Whole genome sequencing of enteroviruses species A to D by high-throughput sequencing: application for viral mixtures. Front Microbiol 9:2339

    Article  PubMed  PubMed Central  Google Scholar 

  12. Bessaud M, Sadeuh-Mba SA, Joffret ML, Razafindratsimandresy R, Polston P, Volle R et al (2016) Whole genome sequencing of enterovirus species C isolates by high-throughput sequencing: development of generic primers. Front Microbiol 7:1294

    Article  PubMed  PubMed Central  Google Scholar 

  13. Oberste MS, Maher K, Kilpatrick DR, Flemister MR, Brown BA, Pallansch MA (1999) Typing of human enteroviruses by partial sequencing of VP1. J Clin Microbiol 37(5):1288–1293

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Oberste MS, Maher K, Kilpatrick DR, Pallansch MA (1999) Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73(3):1941–1948

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Karrasch M, Fischer E, Scholten M, Sauerbrei A, Henke A, Renz DM et al (2016) A severe pediatric infection with a novel enterovirus A71 strain, Thuringia, Germany. J Clin Virol 84:90–95

    Article  PubMed  Google Scholar 

  16. Bessaud M, Razafindratsimandresy R, Nougairede A, Joffret ML, Deshpande JM, Dubot-Peres A et al (2014) Molecular comparison and evolutionary analyses of VP1 nucleotide sequences of new African human enterovirus 71 isolates reveal a wide genetic diversity. PLoS One 9(3):e90624

    Article  PubMed  PubMed Central  Google Scholar 

  17. Volle R, Bailly JL, Mirand A, Pereira B, Marque-Juillet S, Chambon M et al (2014) Variations in cerebrospinal fluid viral loads among enterovirus genotypes in patients hospitalized with laboratory-confirmed meningitis due to enterovirus. J Infect Dis 210(4):576–584

    Article  PubMed  Google Scholar 

  18. Cobbin JCA, Britton PN, Burrell R, Thosar D, Selvakumar K, Eden JS et al (2018) A complex mosaic of enteroviruses shapes community-acquired hand, foot and mouth disease transmission and evolution within a single hospital. Virus Evol. 4(2):vey020

    Article  PubMed  PubMed Central  Google Scholar 

  19. Majumdar M, Martin J (2018) Detection by direct next generation sequencing analysis of emerging enterovirus D68 and C109 strains in an environmental sample from Scotland. Front Microbiol 9:1956

    Article  PubMed  PubMed Central  Google Scholar 

  20. Cabrerizo M, Trallero G, Simmonds P (2014) Recombination and evolutionary dynamics of human echovirus 6. J Med Virol 86(5):857–864

    Article  PubMed  Google Scholar 

  21. Ji T, Guo Y, Huang W, Shi Y, Xu Y, Tong W et al (2018) The emerging sub-genotype C2 of CoxsackievirusA10 associated with hand, foot and mouth disease extensively circulating in mainland of China. Sci Rep 8(1):13357

    Article  PubMed  PubMed Central  Google Scholar 

  22. Henquell C, Mirand A, Richter J, Schuffenecker I, Bottiger B, Diedrich S et al (2013) Phylogenetic patterns of human coxsackievirus B5 arise from population dynamics between two genogroups and reveal evolutionary factors of molecular adaptation and transmission. J Virol 87(22):12249–12259

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Baicus A (2015) Rapid communication—polio vaccine coverage in the acute flaccid paralysis (Afp) cases in Romania. Roum Arch Microbiol Immunol. 74(1–2):65–67

    PubMed  Google Scholar 

  24. Anonymous. WHO vaccine-preventable diseases: monitoring system 2019 global summary. https://apps.who.int/immunization_monitoring/globalsummary/timeseries/tscoveragepol3.html. Accessed 23 Nov 2019

  25. Hamisu AW, Blake IM, Sume G, Braka F, Jimoh A, Dahiru H et al (2020) Characterizing environmental surveillance sites in Nigeria and their sensitivity to detect poliovirus and other enteroviruses. J Infect Dis. https://doi.org/10.1093/infdis/jiaa175

    Article  PubMed  PubMed Central  Google Scholar 

  26. Roberts JA, Hobday LK, Ibrahim A, Thorley BR (2020) Australian National Enterovirus Reference Laboratory annual report, 2018. Commun Dis Intell (2018). https://doi.org/10.33321/cdi.2020.44.26

    Article  Google Scholar 

  27. Zoni R, Mezzetta S, Affanni P, Colucci ME, Fiore S, Fontana S et al (2019) Poliovirus and non-polio-enterovirus environmental surveillance in Parma within the “Global Polio Eradication Program” (GPEI). Acta Biomed. 90(9-S):95–97

    PubMed  PubMed Central  Google Scholar 

  28. Ivanova OE, Yarmolskaya MS, Eremeeva TP, Babkina GM, Baykova OY, Akhmadishina LV et al (2019) Environmental surveillance for poliovirus and other enteroviruses: long-term experience in Moscow, Russian Federation, 2004–2017. Viruses. 11(5):424

    Article  CAS  PubMed Central  Google Scholar 

  29. Chen P, Liu Y, Wang H, Liu G, Lin X, Zhang W et al (2020) Environmental surveillance complements the case-based AFP surveillance in the polio endgame strategy. Appl Environ Microbiol 86(15):e00702-20

    Article  PubMed  PubMed Central  Google Scholar 

  30. Coulliette-Salmond AD, Alleman MM, Wilnique P, Rey-Benito G, Wright HB, Hecker JW et al (2019) Haiti poliovirus environmental surveillance. Am J Trop Med Hyg 101(6):1240–1248

    Article  PubMed  PubMed Central  Google Scholar 

  31. Bessaud M, Pillet S, Ibrahim W, Joffret ML, Pozzetto B, Delpeyroux F et al (2012) Molecular characterization of human enteroviruses in the Central African Republic: uncovering wide diversity and identification of a new human enterovirus A71 genogroup. J Clin Microbiol 50(5):1650–1658

    Article  PubMed  PubMed Central  Google Scholar 

  32. Bisseux M, Colombet J, Mirand A, Roque-Afonso AM, Abravanel F, Izopet J et al (2018) Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015. Euro Surveill. 23(7):17

    Article  PubMed Central  Google Scholar 

  33. Andres C, Vila J, Gimferrer L, Pinana M, Esperalba J, Codina MG et al (2019) Surveillance of enteroviruses from paediatric patients attended at a tertiary hospital in Catalonia from 2014 to 2017. J Clin Virol 110:29–35

    Article  PubMed  Google Scholar 

  34. Cinek O, Kramna L, Mazankova K, Kunteova K, Chuda K, E CJC et al (2019) Virus genotyping by massive parallel amplicon sequencing: adenovirus and enterovirus in the Norwegian MIDIA study. J Med Virol 91(4):606–614

    CAS  PubMed  Google Scholar 

  35. Bubba L, Broberg EK, Jasir A, Simmonds P, Harvala H (2020) Enterovirus study c. Circulation of non-polio enteroviruses in 24 EU and EEA countries between 2015 and 2017: a retrospective surveillance study. Lancet Infect Dis. 20(3):350–361

    Article  PubMed  Google Scholar 

  36. Pogka V, Emmanouil M, Labropoulou S, Voulgari-Kokota A, Angelakis E, Mentis AF (2020) Molecular characterization of enteroviruses among hospitalized patients in Greece, 2013–2015. J Clin Virol 127:104349

    Article  CAS  PubMed  Google Scholar 

  37. Richter J, Tryfonos C, Christodoulou C (2019) Molecular epidemiology of enteroviruses in Cyprus 2008–2017. PLoS One 14(8):e0220938

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Baicus A, Persu A, Dinu S, Joffret ML, Delpeyroux F, Oprisan G (2011) The frequency and biodiversity of poliovirus and non-polio enterovirus strains isolated from healthy children living in a limited area in Romania. Arch Virol 156(4):701–706

    Article  CAS  PubMed  Google Scholar 

  39. Muslin C, Mac Kain A, Bessaud M, Blondel B, Delpeyroux F (2019) Recombination in enteroviruses, a multi-step modular evolutionary process. Viruses 11(9):859

    Article  CAS  PubMed Central  Google Scholar 

  40. Kyriakopoulou Z, Pliaka V, Amoutzias GD, Markoulatos P (2015) Recombination among human non-polio enteroviruses: implications for epidemiology and evolution. Virus Genes 50(2):177–188

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

The investigations were funded by the National Authority of Scientific Research and Innovation, Romania, Core Project PN 16 39 01 03/ 2016-2017. Collaborators: Truica C, Pop R, Zorescu C, Ibrahim S, Leahu D, Secarea A, Solomon Chear T from Public Health Authorities, Călărași, Maramures, Suceava, Constanța, Satu Mare, Tulcea, Bucharest, Romania.

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Correspondence to Anda Baicus.

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Handling Editor: Tim Skern.

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Baicus, A., Joffret, ML., Bessaud, M. et al. Reinforced poliovirus and enterovirus surveillance in Romania, 2015–2016. Arch Virol 165, 2627–2632 (2020). https://doi.org/10.1007/s00705-020-04772-7

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  • DOI: https://doi.org/10.1007/s00705-020-04772-7

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