Skip to main content
SpringerLink
Log in

Genotype-dependent efficiency of endosperm development in culture of selected cereals: histological and ultrastructural studies

  • Original Article
  • Published:
Protoplasma Aims and scope Submit manuscript

Abstract

The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7–10 days post-anthesis developed well on MS medium supplemented with auxins and/or cytokinins. The efficiency of endosperm response was highly genotype-dependent and best in two winter cultivars of hexaploid species. The pathways of development and proliferation were very similar among the selected species and cultivars. Histological and scanning electron microscope (SEM) analysis revealed that only the part of the endosperm not touching the medium surface continued growth and development, resulting in swelling. The central part of swollen regions was composed mainly of cells containing many large starch grains. The peripheric parts of developed endosperm consisted of highly vacuolated cells and small cells with dense cytoplasm. SEM showed that cells from the swollen region were covered partially with a membraneous structure. Transmission electron microscope studies of cells from the outer part of the developing region showed features typical for cell activity connected with lipid metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Bajaj YPS, Saini SS, Bidani M (1980) Production of triploid plants from the immature and mature endosperm cultures of rice. Theor Appl Genet 58:17–18

    Google Scholar 

  • Becraft PW (2001) Cell fate specification in the cereal endosperm. Cell Dev Biol 12:387–394

    Article  CAS  Google Scholar 

  • Birsin MA, Özgen M (2004) A comparison of callus induction and plant regeneration from different embryo explants of triticale (Triticosecale Wittmack). Cell Mol Biol Lett 9:353–361

    PubMed  Google Scholar 

  • Chauhan H, Desai SA, Khurana P (2007) Comparative analysis of the differential regeneration response of various genotypes of Triticum aestivum, Triticum durum and Triticum dicoccum. Plant Cell Tiss Org Cult 91:191–199

    Article  CAS  Google Scholar 

  • Evers AD (1969) Development of the endosperm of wheat. Ann Bot 34:547–555

    Google Scholar 

  • Góralski G, Popielarska M, Ślesak H, Siwińska D, Batycka M (2005) Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro. Acta Biol Cracov Ser Bot 47:121–128

    Google Scholar 

  • Hoshino Y, Miyashita T, Thomas TD (2011) In vitro culture of endosperm and its application in plant breeding: approaches to polyploidy breeding. Sci Hort 130:1–8

    Article  CAS  Google Scholar 

  • Kaltsikes PJ, Roupakias DG, Thomas JB (1975) Endosperm abnormalities in Triticum-Secale combination I. Triticosecale and its parental species. Can J Bot 53:2050–2067

    Article  Google Scholar 

  • Kinoshita T (2007) Reproductive barrier and genomic imprinting in the endosperm of flowering plants. Genes Genet Syst 82:177–186

    Article  PubMed  CAS  Google Scholar 

  • Kranz E, von Wiegen P, Quader H, Lörz H (1998) Endosperm development after fusion of isolated, single maize sperm and central cell in vitro. Plant Cell 10:511–524

    PubMed  CAS  Google Scholar 

  • Lai KS, Yusoff K, Maziah M (2011) Extracellular matrix as the early structural marker for Centella asiatica embryogenic tissues. Biol Plant 55:549–553

    Article  CAS  Google Scholar 

  • Li ChY, Li WH, Li Ch, Gaudet DA, Laroche A, Cao LP, Lu ZX (2010) Starch synthesis and programmed cell death during endosperm development in triticale (× Triticosecale Wittmack). J Integr Plant Biol 52:602–615

    PubMed  CAS  Google Scholar 

  • Mares DJ, Norstog K, Stone BA (1975) Early stages in the development of wheat endosperm. I. The change from free nuclear to cellular endosperm. Aust J Bot 23:311–326

    Article  Google Scholar 

  • Mares DJ, Stone BA, Jeffery C, Norstog K (1977) Early stages in the development of wheat endosperm. II. Ultrastructural observations on cell wall formation. Aust J Bot 25:599–613

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:437–497

    Article  Google Scholar 

  • Nakano H, Tashiro T, Maeda E (1975) Plant differentiation in callus tissue induced from immature endosperm of Oryza sativa L. Pflanzenphysiol 76:444–449

    Google Scholar 

  • Olsen O-A (2004) Nuclear endosperm development in cereals and Arabidopsis thaliana. Plant Cell 16:214–227

    Article  Google Scholar 

  • Popielarska M, Ślesak H, Góralski G (2006) Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit (Actinidia deliciosa cv. Hayward). Acta Biol Cracov Ser Bot 48:97–104

    Google Scholar 

  • Popielarska-Konieczna M, Kozieradzka-Kiszkurno M, Świerczyńska J, Góralski G, Ślesak H, Bohdanowicz J (2008a) Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture. Plant Cell Rep 27(7):1137–1145

    Article  PubMed  CAS  Google Scholar 

  • Popielarska-Konieczna M, Kozieradzka-Kiszkurno M, Świerczyńska J, Góralski G, Ślesak H, Bohdanowicz J (2008b) Are extracellular matrix surface network components involved in signaling and protective function? Plant Signal Behav 3:707–709

    Article  PubMed  Google Scholar 

  • Popielarska-Konieczna M, Marcińska I, Nowakowski P (2009) Preliminary studies on isolated endosperm of selected wheat (Triticum L.) species cultured in vitro. Adv Agric Sci Probl Issues 534:241-241 (in polish with English summary)

    Google Scholar 

  • Sabelli PA, Larkins BA (2009) The contribution of cell cycle regulation to endosperm development. Sex Plant Reprod 22:207–219

    Article  PubMed  Google Scholar 

  • Sehgal CB (1974) Growth of barley and wheat endosperm in cultures. Curr Sci 4:38–40

    Google Scholar 

  • Smart MG, O’Brien TP (1983) The development of the wheat embryo in relation to the neighbouring tissues. Protoplasma 114:1–13

    Article  Google Scholar 

  • Sun CS and Chu CC (1981) The induction of endosperm plantlets and their ploidy of barley in vitro. Acta Bot Sin 23:265 (English summary)

    Google Scholar 

  • Thomas TD, Chaturvedi R (2008) Endosperm culture: a novel method for triploid plant production. Plant Cell Tiss Organ Cult 93:1–14

    Article  Google Scholar 

  • van der Schoot C, Paul LK, Paul SB, Rinne PLH (2011) Plant lipid bodies and cell-cell signaling. A new role for an old organelle? Plant Sign Behav 6:1732–1738

    Article  Google Scholar 

  • Wanner G, Theimer RR (1978) Membranous appendices of spherosomes (oleosomes). Possible role in fat utilization in germinating oil seeds. Planta 140:163–169

    Article  Google Scholar 

  • Zhou Z, Wang L, Li J, Song X, Yang Ch (2009) Study on programmed cell death and dynamic changes of starch accumulation in pericarp cells of Triticum aestivum L. Protoplasma 236:49–58

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

SEM images were made in the Laboratory of Scanning Electron Microscopy for Biological and Geological Sciences, Institute of Zoology, Jagiellonian University. Some of the equipment for this laboratory was provided by the Foundation for Polish Science, Program Subin 94.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Plant Cytology and Embryology, Jagiellonian University, Grodzka St. 52, 31-044, Cracow, Poland

    Marzena Popielarska-Konieczna, Monika Tuleja & Halina Ślesak

  2. Department of Plant Cytology and Embryology, University of Gdańsk, Kładka St. 24, 80-822, Gdańsk, Poland

    Małgorzata Kozieradzka-Kiszkurno & Jerzy Bohdanowicz

  3. Department of Ecology, Polish Academy of Sciences, Lubicz St. 46, 32-512, Cracow, Poland

    Paweł Kapusta

  4. The F. Górski Institute of Plant Physiology of Polish Academy of Sciences, Niezapominajek St. 21, 30-239, Cracow, Poland

    Izabela Marcińska

Authors
  1. Marzena Popielarska-Konieczna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Małgorzata Kozieradzka-Kiszkurno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monika Tuleja
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Halina Ślesak
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paweł Kapusta
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Izabela Marcińska
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jerzy Bohdanowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marzena Popielarska-Konieczna.

Additional information

Handling Editor: Alexander Schulz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Popielarska-Konieczna, M., Kozieradzka-Kiszkurno, M., Tuleja, M. et al. Genotype-dependent efficiency of endosperm development in culture of selected cereals: histological and ultrastructural studies. Protoplasma 250, 361–369 (2013). https://doi.org/10.1007/s00709-012-0419-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00709-012-0419-1

Keywords

Search

Navigation