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A study of Pt(II)–phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI–MS, circular dichroism, and computational docking

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Abstract

The binding interactions of a series of square-planar platinum(II)–phenanthroline complexes of the type [Pt(PL)(AL)]2+ [where PL = variously methyl-substituted 1,10-phenanthroline (phen) and AL = ethane-1,2-diamine (en)] were assessed with a G-quadruplex DNA (5′-TTG GGG GT-3′, G4DNA) and a double-stranded DNA (5′-CGC GAA TTC GCG-3′, dsDNA) sequence by ESI–MS. The results indicate a strong correlation between G4DNA affinity and increasing phenanthroline methyl substitution. Circular dichroism (CD) spectroscopy and molecular docking studies also support the finding that increased substitution of the phenanthroline ligand increased selectivity for G4DNA.

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ESI-MS was used to probe the interaction of a range of square-planar Pt(II)–phenanthroline complexes with double-stranded and G-quadruplex DNA.

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Acknowledgements

Thanks to WSU Mass Spectrometry Facility for access to the ToF mass spectrometer. Thanks also to WSU Summer Scholarship students Yuliani Yuliani and Sandy Craze for contributions to the experimental component and Dr Elise Wright for helpful discussions. D.L.A. was supported by an Australian Postgraduate Award with WSU top up.

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Authors and Affiliations

  1. Nanoscale Organisation and Dynamics Group, School of Science, Western Sydney University, Penrith, NSW, 2751, Australia

    Dale L. Ang & Janice R. Aldrich-Wright

  2. School of Medicine, Western Sydney University, Locked Bag 1797, Penrith South DC, NSW, 2751, Australia

    David G. Harman & Janice R. Aldrich-Wright

  3. School of Chemistry and Molecular Bioscience, University of Wollongong, Northfields Avenue, Wollongong, 2522, Australia

    Celine Kelso, Jennifer L. Beck & Stephen F. Ralph

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  1. Dale L. Ang
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Ang, D.L., Kelso, C., Beck, J.L. et al. A study of Pt(II)–phenanthroline complex interactions with double-stranded and G-quadruplex DNA by ESI–MS, circular dichroism, and computational docking. J Biol Inorg Chem 25, 429–440 (2020). https://doi.org/10.1007/s00775-020-01773-4

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