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Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo

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Abstract

Clinical application of ozone gas has been shown to arrest the progression of dentinal caries in children. In this study, we compare the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children. Forty children, each with at least two open occlusal carious lesions, were enrolled in the study. Two teeth were chosen randomly. In one lesion, overlying soft biological material was removed, whilst the other lesion was not excavated. Cavities were rinsed with sterile water and dried with air. A standardised sample was taken from the mesial part of each lesion. Then, gaseous ozone (HealOzone) or 1% chlorhexidine gel (Corsodyl) was applied for 30 s on both lesions of 20 children each, and a second sample was taken from the distal part of each lesion. The anaerobic microbiota was cultivated; the number of colony forming units was calculated per milligram sample. The two-sided paired t test showed no significant (P > 0.05) differences in the reduction of total bacterial counts per milligram comparing samples before and after ozone or chlorhexidine application. The tests also showed no statistically significant difference whether the superficial decayed dentine had been removed before ozone or with chlorhexidine treatment or not. It can be concluded that gaseous ozone or chlorhexidine gel application for 30 s to deep occlusal carious cavities had no significant immediate antimicrobial effects whether the superficial decayed layers dentine were removed or not.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Preventive Dentistry and Oral Microbiology, School of Dental Medicine, University of Basel, Basel, Switzerland

    Irmgard Hauser-Gerspach & Jürg Meyer

  2. Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland

    Victoria Pfäffli-Savtchenko, Jan Eric Dähnhardt & Adrian Lussi

  3. Klinik für Zahnerhaltung, Präventiv–und Kinderzahnmedizin, Universität Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland

    Adrian Lussi

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  1. Irmgard Hauser-Gerspach
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  2. Victoria Pfäffli-Savtchenko
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  3. Jan Eric Dähnhardt
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  4. Jürg Meyer
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  5. Adrian Lussi
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Correspondence to Adrian Lussi.

Additional information

Irmgard Hauser-Gerspach and Victoria Pfäffli-Savtchenko contributed equally.

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Hauser-Gerspach, I., Pfäffli-Savtchenko, V., Dähnhardt, J.E. et al. Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo. Clin Oral Invest 13, 287–291 (2009). https://doi.org/10.1007/s00784-008-0234-4

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  • DOI: https://doi.org/10.1007/s00784-008-0234-4

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