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The mineralizing effect of zinc oxide-modified hydroxyapatite-based sealer on radicular dentin

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Abstract

Objective

The aim of this study was to evaluate the remineralization ability of three endodontic sealer materials at different root dentin regions.

Material and methods

Cervical, medial, and apical root dentin surfaces were treated with two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypatite) or zinc oxide (oxipatite); an epoxy resin-based canal sealer, AH Plus; and gutta-percha. Remineralization, at the inner and outer zones of dentin disk surfaces, was studied by nanohardness (Hi) and Raman analysis. Nanoroughness and collagen fibrils width measurements were performed. Numerical data, at 24 h or 12 m, were analyzed by ANOVA and Student-Newman-Keuls (p < 0.05).

Results

At the outer and inner zones of the cervical dentin treated with oxipatite, the highest Hi after 12 m of immersion was achieved. The same group showed the highest intensity of phosphate peak, markers for calcification and crystallinity. Nanoroughness was lower and fibril diameter was higher at the inner zone of the dentin treated with oxipatite. Dentin mineralization occurred in every region of the root dentin treated with oxipatite and calcypatite, especially at the inner zone of the dentin after 12 m.

Conclusions

Oxipatite reinforced the inner root zone at any third of the radicular dentin, by increasing both nanohardness and remineralization. When using calcypatite, the highest nanohardness was found at the apical third of the inner root dentin, but the lowest mechanical performance was obtained at the cervical and the medial thirds of the roots. Therefore, application of oxipatite as sealing cement of root canals is recommended.

Clinical relevance

Oxipatite, when used as an endodontic sealing material, strengthens radicular dentin.

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Acknowledgements

Project MAT2017-85999-P MINECO/AEI/FEDER/UE supported by the Ministry of Economy and Competitiveness and European Regional Development Fund.

Funding

Project MAT2017-85999-P MINECO/AEI/FEDER/UE supported by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER).

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Authors and Affiliations

  1. Research Institute IBS, Dental School, University of Granada. Colegio Máximo, Campus de Cartuja s/n, 18017, Granada, Spain

    Manuel Toledano, Esther Muñoz-Soto, Fátima S. Aguilera, Estrella Osorio, Manuel Toledano-Osorio & Raquel Osorio

  2. Biomaterials Department, University of La Havana, San Lázaro y L. Municipio Plaza de la Revolución La Havana, Havana, Cuba

    Mayra C. Pérez-Álvarez & José AD. García-Menocal

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Toledano, M., Muñoz-Soto, E., Aguilera, F.S. et al. The mineralizing effect of zinc oxide-modified hydroxyapatite-based sealer on radicular dentin. Clin Oral Invest 24, 285–299 (2020). https://doi.org/10.1007/s00784-019-02938-5

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