Skip to main content
SpringerLink
Log in

Comparison of the Roche Cobas® 4800 HPV assay to Roche Amplicor for detection of high-risk human papillomavirus

  • Original Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

Roche Amplicor HPV (AMP) had previously been used for detection of high-risk human papillomavirus (HR-HPV) in epidemiological and clinical studies. As this assay is no longer available, we compared its performance using PreservCyt samples from women aged of 18–24 years attending for routine cervical cytology screening to Roche Cobas® 4800 (Cobas) to determine if subsequent studies could continue using the Cobas assay. Overall 507 samples were tested on Cobas and compared to previous AMP results, with discrepant samples tested on Roche Linear Array. Results: Overall, agreement between the Cobas and AMP for the presence of HR HPV types was very high (κ = 0.81) (95 % CI: 0.76 - 0.87) with percentage agreement of 91.57 %. Cobas is comparable to AMP for the detection of HR-HPV types in a community recruited cohort of healthy women.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tabrizi SN et al (2014) Assessment of herd immunity and cross-protection after a human papillomavirus vaccination programme in Australia: a repeat cross-sectional study. Lancet Infect Dis 14(10):958–66

    Article  PubMed  Google Scholar 

  2. Phillips S et al (2015) Comparison of the Roche Cobas((R)) 4800 HPV assay to Digene Hybrid Capture 2, Roche Linear Array and Roche Amplicor for Detection of High-Risk Human Papillomavirus Genotypes in Women undergoing treatment for cervical dysplasia. J Clin Virol 62:63–5

    Article  CAS  PubMed  Google Scholar 

  3. Nygard M et al (2014) Comparative effectiveness study on human papillomavirus detection methods used in the cervical cancer screening programme. BMJ Open 4(1), e003460

    Article  PubMed  PubMed Central  Google Scholar 

  4. Tabrizi SN et al (2012) Comparison of PapType to Digene Hybrid Capture 2, Roche linear array, and Amplicor for detection of high-risk human papillomavirus genotypes in women with previous abnormal pap smears. J Clin Microbiol 50(8):2796–8

    Article  PubMed  PubMed Central  Google Scholar 

  5. Stoler MH et al (2012) The interplay of age stratification and HPV testing on the predictive value of ASC-US cytology. Results from the ATHENA HPV study. Am J Clin Pathol 137(2):295–303

    Article  PubMed  Google Scholar 

  6. Wright, T.C., Jr., et al. (2012) The ATHENA human papillomavirus study: design, methods, and baseline results. Am J Obstet Gynecol. 206(1): p. 46 e1-46 e11

  7. Mackinnon A (2000) A spreadsheet for the calculation of comprehensive statistics for the assessment of diagnostic tests and inter-rater agreement. Comput Biol Med 30(3):127–34

    Article  CAS  PubMed  Google Scholar 

  8. Stevens MP et al (2007) Comparison of the Digene Hybrid Capture 2 assay and Roche AMPLICOR and LINEAR ARRAY human papillomavirus (HPV) tests in detecting high-risk HPV genotypes in specimens from women with previous abnormal Pap smear results. J Clin Microbiol 45(7):2130–7

    Article  PubMed  PubMed Central  Google Scholar 

  9. Gage JC et al (2012) Comparison of the cobas Human Papillomavirus (HPV) test with the hybrid capture 2 and linear array HPV DNA tests. J Clin Microbiol 50(1):61–5

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women’s Hospital, Parkville, Victoria, Australia

    S. Phillips, A. M. Cornall, D. A. Machalek, S. M. Garland & S. N. Tabrizi

  2. Department of Microbiology and Infectious Diseases, The Royal Women’s Hospital, Bio 21 Institute, Parkville, 3052, Australia

    S. Phillips

  3. Murdoch Childrens Research Institute, Parkville, Victoria, 3052, Australia

    S. Phillips, A. M. Cornall, D. A. Machalek, S. M. Garland & S. N. Tabrizi

  4. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia

    S. M. Garland & S. N. Tabrizi

  5. Family Planning New South Wales, Ashfield, NSW, Australia

    D. Bateson

  6. Family Planning Western Australia, Northbridge, WA, Australia

    M. Garefalakis

Authors
  1. S. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Cornall
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Machalek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. M. Garland
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Bateson
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Garefalakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. N. Tabrizi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Phillips.

Ethics declarations

Funding

The funding for this study was provided by departmental funding.

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This study was endorsed by the Royal Women’s Hospital Human Research and Ethics Committees. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

For this retrospective study formal consent was not required from participants. This article does not contain any studies with animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Phillips, S., Cornall, A.M., Machalek, D.A. et al. Comparison of the Roche Cobas® 4800 HPV assay to Roche Amplicor for detection of high-risk human papillomavirus. Eur J Clin Microbiol Infect Dis 35, 1305–1307 (2016). https://doi.org/10.1007/s10096-016-2665-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-016-2665-1

Keywords

Search

Navigation