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Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae

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Abstract

The spread of multidrug-resistant (MDR) Klebsiella pneumoniae in the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic–based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of an outbreak involving KPC-3-producing K. pneumoniae ST147 isolates. Our results further support that attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy can provide enough information to support early and adequate infection control measures and therapeutic choices in the context of nosocomial outbreaks and hospital surveillance.

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Funding

This work received financial support from Applied Molecular Biosciences Unit UCIBIO, which is supported by national funds from Fundação para a Ciência e a Tecnologia (FCT)/MCTES [UID/Multi/04378/2020]. Ângela Novais is supported by national funds through FCT, I.P., in the context of the transitional norm [DL57/2016/CP1346/CT0032]. Liliana Silva and Carla Rodrigues were supported by FCT and Programa Operacional Capital Humano (POCH) through grants SFRH/BD/88028/2012 and SFRH/BD/84341/2012, respectively.

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Authors and Affiliations

  1. UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal

    Liliana Silva, Carla Rodrigues, Ângela Novais & Luísa Peixe

  2. ESALD, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal

    Liliana Silva

  3. Biodiversity & Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France

    Carla Rodrigues

  4. CHVNG/E, Serviço de Patologia Clínica, Centro Hospitalar de Vila Nova de Gaia/Espinho, Vila Nova de Gaia, Portugal

    Agostinho Lira, Mariana Leão, Margarida Mota & Paulo Lopes

Authors
  1. Liliana Silva
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  2. Carla Rodrigues
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  3. Agostinho Lira
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  4. Mariana Leão
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  5. Margarida Mota
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  6. Paulo Lopes
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  7. Ângela Novais
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  8. Luísa Peixe
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Corresponding authors

Correspondence to Ângela Novais or Luísa Peixe.

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Silva, L., Rodrigues, C., Lira, A. et al. Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae. Eur J Clin Microbiol Infect Dis 39, 2471–2475 (2020). https://doi.org/10.1007/s10096-020-03956-y

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  • DOI: https://doi.org/10.1007/s10096-020-03956-y

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