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CD147 expression in peritoneal injury

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Abstract

Background

Peritoneal injury is an important cause of technical failure of long-term peritoneal dialysis (PD). Encapsulating peritoneal sclerosis (EPS) is a severe complication of long-term PD with potentially life threatening consequences. CD147 is a glycoprotein with diverse functions including modulation of extracellular matrix via induction of matrix metalloproteinases, cell adhesion, and regulation of immune reactions. We hypothesized that CD 147 plays a role in the peritoneal cavity.

Methods

In this retrospective study, we localized CD147 by immunohistochemistry in peritoneal biopsies from uremic patients not on PD (n = 8), on PD without signs of EPS (n = 7), and in biopsies in patients with the diagnosis of EPS (n = 7). Double immunofluorescence was used to co-localize α-smooth-muscle actin (α-SMA) and CD147 in selected biopsies from each group. Expression was scored semi-quantitatively.

Results

In biopsies from uremic controls, CD147 was prominently expressed in mesothelial cells, focally between fat cells and by some perivascular cells. In patients on PD, a similar distribution was present (although mesothelium was rarely conserved), with some focal accentuation. In EPS, layers of fibroblastic cells were positive for CD147. EPS biopsies demonstrated a significantly higher score in a blinded evaluation, compared to uremic patients. Cells expressing CD147 were α-SMA positive myofibroblasts as demonstrated by double immunofluorescence. Mean CD147 scores did not differ between patients with different transporter status.

Conclusions

This is the first study demonstrating CD147 on a major part of fibroblastic cells in EPS. Future studies need to address the role of these cells in this severe complication of long-term PD.

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Acknowledgements

St. S. is supported by a Grant by Fundação Pesquisa e Desenvolvimento Humanitario; and the Else-Kröner Fresenius Stiftung. J.L. and D.K. by the Robert-Bosch Foundation.

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    Authors and Affiliations

    1. Division of Nephrology, University Hospital, Rämistr. 100, 8091, Zurich, Switzerland

      Harald Seeger, Jin Chen, Ilka Edenhofer, Rudolf P. Wüthrich & Stephan Segerer

    2. Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland

      Harald Seeger, Jin Chen & Ilka Edenhofer

    3. Division of General Internal Medicine and Nephrology, Department of Internal Medicine, Robert-Bosch-Hospital, Stuttgart, Germany

      Joerg Latus, Daniel Kitterer & M. Dominik Alscher

    4. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany

      Dagmar Biegger

    5. Division of Nephrology, Dialysis and Transplantation, Kantonsspital, Tellstrasse 25, 5001, Aarau, Switzerland

      Stephan Segerer

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    1. Harald Seeger
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    Correspondence to Stephan Segerer.

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    Conflict of interest

    Stephan Segerer receives benefits from Peripal and Baxter as a consultant.

    Ethical approval

    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee at which the studies were conducted (#322/2009BO1, Ethic committee, Eberhard-Karls University Tuebingen, Germany) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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    All patients had given their written informed consent concerning a scientific work-up of tissues taken during surgery.

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    Harald Seeger and Joerg Latus contributed equally to the study.

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    Seeger, H., Latus, J., Kitterer, D. et al. CD147 expression in peritoneal injury. Clin Exp Nephrol 21, 1097–1104 (2017). https://doi.org/10.1007/s10157-017-1390-0

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