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Testing hypotheses on the origin and dispersion of Limnoperna fortunei (Bivalvia, Mytilidae) in the Iguassu River (Paraná, Brazil): molecular markers in larvae and adults

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Abstract

The invasive bivalve Limnoperna fortunei (Dunker 1857) was introduced in South America in 1991, with the first occurrence in Brazil in 1998. In the Iguassu River, the species was recorded in 2001; however, it is unknown how it was introduced and spread. Adults and larvae were sampled in Iguassu and Paraná Rivers, and the genetic profiles were compared. The species was absent in the upper reaches and only larvae were found, in low densities, in intermediary reaches. The L. fortunei populations from the lower Iguassu River presented no genetic differentiation among themselves, suggesting strong connectivity, and were significantly different from the Paraná River populations, most likely because of Iguassu Falls. Furthermore, the results suggest that the Paraná River represents the source of propagules to the Iguassu River. Generally, no significant differences were observed between the genetic structure inferred from adults and larvae. Only the population from the Iguassu National Park, a lotic environment, differed from the remaining subpopulations. The characterization of genetic profile using larval stages of L. fortunei populations was satisfactory, and represents an important protocol for studying the population genetics of aquatic species with planktonic larval stages.

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Acknowledgments

The authors thank the Companhia Paranaense de Energia—COPEL and Tractebel Energia, Eletric Utilities at which services and R and D projects were conducted, regulated by the Agência Nacional de Energia Elétrica—ANEEL and that provided data to develop this research. Additionally, the authors thank D. Agostinho from Itaipu Binacional Hidroeltric Power Plant for providing samples, Companhia de Saneamento do Paraná—SANEPAR for authorizing the study at Piraquara II Dam and for all field support, colleagues from the Institutos Lactec for collecting samples and colleagues from LEMPE for helping with the molecular protocols and methodology.

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Authors and Affiliations

  1. Instituto de Tecnologia Para o Desenvolvimento - Institutos Lactec, BR 116, Km 98, nº 8813, Curitiba, Paraná, Brazil

    Patricia Dammski Borges

  2. Laboratório de Ecologia Molecular e Parasitologia Evolutiva, Departmento de Zoologia, Universidade Federal do Paraná, Curitiba, Brazil

    Patricia Dammski Borges, Sandra Ludwig & Walter A. Boeger

  3. Curso de Pós-graduação em Ciências Biológicas - Zoologia, UFPR, Curitiba, Brazil

    Patricia Dammski Borges, Sandra Ludwig & Walter A. Boeger

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  1. Patricia Dammski Borges
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  2. Sandra Ludwig
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Correspondence to Patricia Dammski Borges.

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Borges, P.D., Ludwig, S. & Boeger, W.A. Testing hypotheses on the origin and dispersion of Limnoperna fortunei (Bivalvia, Mytilidae) in the Iguassu River (Paraná, Brazil): molecular markers in larvae and adults. Limnology 18, 31–39 (2017). https://doi.org/10.1007/s10201-016-0485-8

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