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Raman spectroscopic study of noncarious cervical lesions

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Abstract

The surface of noncarious cervical lesions (NCCLs) consists of sclerosed dentin. This type of dentin may affect the ability of adhesive restorative materials to bond well to its surface, but little information exists on the chemical nature of this dentin surface and how it may be affected during acidic treatment. The inorganic part of normal dentin and dentin from NCCLs before and after acid conditioning with phosphoric acid or polyacrylic acid was investigated. Ten premolars with NCCLs and four human third molars (control) were used. Replicas of NCCLs were examined using scanning electron microscopy (SEM). Surfaces and longitudinal sections of four NCCLs and control dentin discs were analyzed using Raman spectroscopy. The discs and NCCLs were sectioned, and treated with 35% phosphoric acid or 20% polyacrylic acid/3% aluminum chloride, and Raman spectra obtained. The area under phosphate ν1 of the dentin spectrum was computed to obtain a ratio with the area under the second-order spectrum of a silicon phonon comparative standard. Mean phosphate ν1 and silicon phonon ratios from normal dentin and NCCLs were compared using a linear model with repeated measurements and Tukey’s pairwise tests. Mean ratios from different locations of the NCCLs were compared using one-way analysis of variance (ANOVA) and Tukey’s pairwise tests. SEM micrographs of NCCL surfaces showed variation from relatively smooth with no dentinal tubule openings to surfaces with occluded tubules. The mean phosphate ν1 and silicon phonon ratios for NCCLs were higher than those of normal dentin in all treatment groups (P < 0.05). Ratios from the untreated specimens were higher than those of the polyacrylic acid-treated specimens, and those for the phosphoric acid-treated group were the lowest (P < 0.05). The ratios obtained for the surfaces of NCCLs were higher than those halfway towards the pulp, and those adjacent to the pulp were the lowest (P < 0.05).

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References

  1. JO Grippo (1991) ArticleTitleAbfractions: a new classification of hard tissue lesions of teeth J Esthet Dent 3 14–9 Occurrence Handle1873064

    PubMed  Google Scholar 

  2. JO Grippo M Simring (1995) ArticleTitleDental “erosion” revisited J Am Dent Assoc 126 619–30 Occurrence Handle7759687

    PubMed  Google Scholar 

  3. MJ Tyas (1995) ArticleTitleThe class V lesion – aetiology and restoration Aust Dent J 40 167–70 Occurrence Handle7661762

    PubMed  Google Scholar 

  4. WD Browning WW Brackett RO Gilpatrick (1999) ArticleTitleRetention of microfilled and hybrid resin-based composite in non-carious class 5 lesions: a double-blind, randomized clinical trial Oper Dent 24 26–30 Occurrence Handle10337295

    PubMed  Google Scholar 

  5. LC Levitch JD Bader DA Shugars HO Heymann (1994) ArticleTitleNon-carious cervical lesions J Dent 22 195–207 Occurrence Handle10.1016/0300-5712(94)90107-4 Occurrence Handle7962894

    Article  PubMed  Google Scholar 

  6. JS Hand RJ Hunt JW Reinhardt (1986) ArticleTitleThe prevalence and treatment implications of cervical abrasion in the elderly Gerodontics 2 167–70 Occurrence Handle3468033

    PubMed  Google Scholar 

  7. ES Duke J Lindemuth (1990) ArticleTitlePolymeric adhesion to dentin: contrasting substrates Am J Dent 3 264–70 Occurrence Handle2095806

    PubMed  Google Scholar 

  8. M Yoshiyama J Masada A Uchida H Ishida (1989) ArticleTitleScanning electron microscope characterization of sensitive vs insensitive human radicular dentin J Dent Res 68 1398–402

    Google Scholar 

  9. IA Mjör (1987) Reaction patterns of dentin A Thylstrup SA Leach V Qvist (Eds) Dentin and dentin reactions in the oral cavity IRL Oxford 27–31

    Google Scholar 

  10. P Tramini B Pelissier J Valcarcel B Bonnet (2000) ArticleTitleA Raman spectroscopic investigation of dentin and enamel structures modified by lactic acid Caries Res 34 233–40 Occurrence Handle10.1159/000016596 Occurrence Handle10867422

    Article  PubMed  Google Scholar 

  11. H Tsuda J Arends (1993) ArticleTitleRaman spectra of human dental calculus J Dent Res 72 1609–13 Occurrence Handle8254131

    PubMed  Google Scholar 

  12. H Tsuda J Arends (1994) ArticleTitleOrientational micro-Raman spectroscopy on hydroxyapatite single crystals and human enamel crystallites J Dent Res 73 1703–10 Occurrence Handle7983256

    PubMed  Google Scholar 

  13. P Spencer Y Wang MP Walker DM Wieliczka JR Swafford (2000) ArticleTitleInterfacial chemistry of the dentin/adhesive bond J Dent Res 79 1458–63 Occurrence Handle11005728

    PubMed  Google Scholar 

  14. M Suzuki H Kato S Wakumoto (1991) ArticleTitleVibrational analysis by Raman spectroscopy of the interface between dental adhesive resin and dentin J Dent Res 70 1092–7 Occurrence Handle2066492

    PubMed  Google Scholar 

  15. B Van Meerbeek H Mohrbacher JP Celis JR Roos M Braem P Lambrechts G Vanherle (1993) ArticleTitleChemical characterization of the resin-dentin interface by micro-Raman spectroscopy J Dent Res 72 1423–8 Occurrence Handle8408885

    PubMed  Google Scholar 

  16. J Xu I Stangel IS Butler DF Gilson (1997) ArticleTitleAn FT-Raman spectroscopic investigation of dentin and collagen surfaces modified by 2-hydroxyethylmethacrylate J Dent Res 76 596–601 Occurrence Handle9042083

    PubMed  Google Scholar 

  17. H Tsuda J Ruben J Arends (1996) ArticleTitleRaman spectra of human dentin mineral Eur J Oral Sci 104 123–31 Occurrence Handle8804900

    PubMed  Google Scholar 

  18. FR Tay SM Kwong A Atthagarun NM King HK Yip KM Moulding DH Pashley (2000) ArticleTitleBonding of a self-etching primer to noncarious cervical sclerotic dentin: interfacial ulrastructure and micro-tensile bond strength evaluation J Adhes Dent 2 9–28 Occurrence Handle11317411

    PubMed  Google Scholar 

  19. DGA Nelson BE Williamson (1982) ArticleTitleLow-temperature laser Raman spectroscopy of synthetic carbonate apatite and dental enamel Aust J Chem 35 715–27

    Google Scholar 

  20. SM Kwong FR Tay HK Yip LH Kei DH Pashley (2000) ArticleTitleAn ultrastructural study of the application of dentin adhesives to acid-conditioned sclerotic dentin J Dent 28 515–28 Occurrence Handle10.1016/S0300-5712(00)00032-4 Occurrence Handle10960756

    Article  PubMed  Google Scholar 

  21. AJ Gwinnett MD Jendresen (1978) ArticleTitleMicromorphologic features of cervical erosion after acid conditioning and its relation with composite resin J Dent Res 57 543–9 Occurrence Handle361772

    PubMed  Google Scholar 

  22. I Kaplan HH Mincer EF Harris JS Cloyd (1992) ArticleTitleMicroleakage of composite resin and glass ionomer cement restorations in retentive and nonretentive cervical cavity preparations J Prosthet Dent 68 616–23 Occurrence Handle1403938

    PubMed  Google Scholar 

  23. LV Powell GH Johnson GE Gordon (1995) ArticleTitleFactors associated with clinical success of cervical abrasion/erosion restorations Oper Dent 20 7–13 Occurrence Handle8700767

    PubMed  Google Scholar 

  24. X Lepe GH Johnson LA Mancl (2005) ArticleTitleA 3-year clinical evaluation of two-bottle versus one-bottle dentin adhesives J Am Dent Assoc 136 311–22 Occurrence Handle15819344

    PubMed  Google Scholar 

  25. MF Burrow MJ Tyas (2003) ArticleTitleClinical evaluation of an “all-in-one” bonding system to non-carious cervical lesions – results at 1 year Aust Dent J 48 180–2 Occurrence Handle14640371

    PubMed  Google Scholar 

  26. M Ferrari CL Davidson (1997) ArticleTitleInterdiffusion of a traditional glass ionomer cement into conditioned dentin Am J Dent 10 295–7 Occurrence Handle9590920

    PubMed  Google Scholar 

  27. A Lin NS McIntyre RD Davidson (1992) ArticleTitleStudies on the adhesion of glass-ionomer cements to dentin J Dent Res 71 1836–41 Occurrence Handle1401448

    PubMed  Google Scholar 

  28. GJ Mount (1994) ArticleTitleGlass-ionomer cements: past, present and future Oper Dent 19 82–90 Occurrence Handle9028245

    PubMed  Google Scholar 

  29. R Sakoolnamarka MF Burrow S Prawer MJ Tyas (2000) ArticleTitleMicromorphological investigation of non-carious cervical lesions treated with demineralizing agents J Adhes Dent 2 279–87 Occurrence Handle11317374

    PubMed  Google Scholar 

  30. C Harnirattisai S Inokoshi Y Shimada H Hosoda (1993) ArticleTitleAdhesive interface between resin and etched dentin of cervical erosion/abrasion lesions Oper Dent 18 138–43 Occurrence Handle8152981

    PubMed  Google Scholar 

  31. DH Pashley HJ Andringa GD Derkson ME Derkson SR Kalathoor (1987) ArticleTitleRegional variability in permeability of human dentin Arch Oral Biol 32 519–23 Occurrence Handle3479091

    PubMed  Google Scholar 

  32. JWV van Dijken (2000) ArticleTitleClinical evaluation of three adhesive systems in class V noncarious lesions Dent Mater 16 285–91 Occurrence Handle10.1016/S0109-5641(00)00019-1 Occurrence Handle10831784

    Article  PubMed  Google Scholar 

  33. JW van Dijken (2004) ArticleTitleDurability of three simplified adhesive systems in class V non-carious cervical dentin lesions Am J Dent 17 27–32 Occurrence Handle15241906

    PubMed  Google Scholar 

  34. Burrow MF, Tyas MJ. Comparative clinical evaluation of SE Bond with a single bottle adhesive and RM-GIC – results at 2 years. The Second International Congress on Adhesive Dentistry,Tokyo, Japan,April 22–24, 2005, Abstract P10.

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Authors and Affiliations

  1. School of Dental Science, The University of Melbourne, Victoria, 3010, Australia

    Rangsima Sakoolnamarka, Michael F. Burrow & Martin J. Tyas

  2. Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

    Rangsima Sakoolnamarka

  3. School of Physics, The University of Melbourne, Melbourne, Australia

    Steven Prawer

Authors
  1. Rangsima Sakoolnamarka
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  2. Michael F. Burrow
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  3. Steven Prawer
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  4. Martin J. Tyas
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Correspondence to Michael F. Burrow.

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Sakoolnamarka, R., Burrow, M., Prawer, S. et al. Raman spectroscopic study of noncarious cervical lesions. Odontology 93, 35–40 (2005). https://doi.org/10.1007/s10266-005-0052-y

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  • DOI: https://doi.org/10.1007/s10266-005-0052-y

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