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Regenerative and Resorbable PLA/HA Hybrid Construct for Tendon/Ligament Tissue Engineering

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Abstract

Tendon and ligament shows extremely limited endogenous regenerative capacity. Current treatments are based on the replacement and or augmentation of the injured tissue but the repaired tissue rarely achieve functionality equal to that of the preinjured tissue. To address this challenge, tissue engineering has emerged as a promising strategy. This study develops a regenerative and resorbable hybrid construct for tendon and ligament engineering. The construct is made up by a hollow poly-lactic acid braid with embedded microspheres carrying cells and an anti-adherent coating, with all the parts being made of biodegradable materials. This assembly intends to regenerate the tissue starting from the interior of the construct towards outside while it degrades. Fibroblasts cultured on poly lactic acid and hyaluronic acid microspheres for 6 h were injected into the hollow braid and the construct was cultured for 14 days. The cells thus transported into the lumen of the construct were able to migrate and adhere to the braid fibers naturally, leading to a homogeneous proliferation inside the braid. Moreover, no cells were found on the outer surface of the coating. Altogether, this study demonstrated that PLA/HA hybrid construct could be a promising material for tendon and ligament repair.

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Acknowledgments

This work was supported by AITEX (Textil Research Institute, Alcoi, Alicante, Spain) through the researching contract “Development of braided biomaterials for biomedical applications” and also funded by AEI “RTI2018-095872-B-C21 and C22/ERDF”.

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

The authors declare that they have fulfilled ethical standards.

Author information

Author notes

  1. M. C. Araque-Monrós

    Present address: AIMPLAS, Plastics Technology Centre, Gustave Eiffel, 4, Paterna, 46980, Valencia, Spain

  2. D. M. García-Cruz

    Present address: Center for Vascular Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA

Authors and Affiliations

  1. CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Valencia, Spain

    M. C. Araque-Monrós & M. Monleón-Pradas

  2. Centro de Biomateriales e Ingeniería Tisular, Universitat Politécnica de València, Camino de Vera s/n, 46022, Valencia, Spain

    M. C. Araque-Monrós, D. M. García-Cruz, J. L. Escobar-Ivirico, L. Gil-Santos, M. Monleón-Pradas & J. Más-Estellés

  3. Instituto Universitario de Investigación en Enfermedades Músculo-esqueléticas Universidad Católica de Valencia (San Vicente Mártir), C/Quevedo, 2, 46001, Valencia, Spain

    L. Gil-Santos

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Araque-Monrós, M.C., García-Cruz, D.M., Escobar-Ivirico, J.L. et al. Regenerative and Resorbable PLA/HA Hybrid Construct for Tendon/Ligament Tissue Engineering. Ann Biomed Eng 48, 757–767 (2020). https://doi.org/10.1007/s10439-019-02403-0

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  • DOI: https://doi.org/10.1007/s10439-019-02403-0

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